Intracellular Delivery of Quantum Dots for Live Cell Labeling and Organelle Tracking

Several strategies borrowed from the field of gene delivery are adapted to enhance delivery of semiconductor quantum dots (QDs) to the interior of live cells. To traffic QDs to subcellular organelles, QDs were then derivatized with known peptide localization sequences, demonstrating the ability to t...

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Bibliographic Details
Published inAdvanced materials (Weinheim) Vol. 16; no. 12; pp. 961 - 966
Main Authors Derfus, A. M., Chan, W. C. W., Bhatia, S. N.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 17.06.2004
WILEY‐VCH Verlag
Subjects
Cytometry
Encapsulation
Nanoparticles
Quantum dots (QDs)
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Summary:Several strategies borrowed from the field of gene delivery are adapted to enhance delivery of semiconductor quantum dots (QDs) to the interior of live cells. To traffic QDs to subcellular organelles, QDs were then derivatized with known peptide localization sequences, demonstrating the ability to target QDs to the nucleus and mitochondria (see Figure).
Bibliography:ark:/67375/WNG-2J6F3V0L-W
ArticleID:ADMA200306111
istex:B3CADB5D7304EAE9FF4DBF348937BF6383143B81
We thank Dennis Young for assistance with flow cytometry, Carolan Buckmaster for microinjection, and Mike Sailor for providing facilities for QD synthesis. Funding provided by DARPA-MTO, David and Lucile Packard Foundation, NIH T32 HL07089, NIH NDDK, University of Toronto (WC), the Connaught Foundation (WC), Canadian Foundation for Innovation (WC), and Ontario Innovation Trust (WC).
We thank Dennis Young for assistance with flow cytometry, Carolan Buckmaster for microinjection, and Mike Sailor for providing facilities for QD synthesis. Funding provided by DARPA‐MTO, David and Lucile Packard Foundation, NIH T32 HL07089, NIH NDDK, University of Toronto (WC), the Connaught Foundation (WC), Canadian Foundation for Innovation (WC), and Ontario Innovation Trust (WC).
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ISSN:0935-9648
1521-4095
DOI:10.1002/adma.200306111