Shedding light on betL: pPL2-lux mediated real-time analysis of betL expression in Listeria monocytogenes

We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ A promoter's −10 and −35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed f...

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Published inBioengineered Vol. 7; no. 2; pp. 116 - 119
Main Authors Keane, Shauna M., Culligan, Eamonn P., Hoffmann, Roland F., Gahan, Cormac G.M., Hill, Colin, Snelling, William J., Sleator, Roy D.
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis 02.04.2016
Subjects
Addendum
Bacterial Proteins - genetics
Betaine - metabolism
betL
Carrier Proteins - genetics
Gene Expression Regulation, Bacterial
Listeria
Listeria monocytogenes - genetics
lux
osmotolerance
Listeria
betL
lux
osmotolerance
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Summary:We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ A promoter's −10 and −35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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These authors contributed equally to this work.
ISSN:2165-5979
2165-5987
DOI:10.1080/21655979.2016.1171438