Preliminary characterization of a Kazal-type serine protease inhibitor from Caiman crocodilus yacare plasma

• Published in: Immunopharmacology Vol. 45; no. 1-3; pp. 179 - 183
• Main Authors: Araujo, Mariana S, Nunes, Viviane A, Gozzo, Andrezza J, Sampaio, Misako U, Auerswald, Ennes, Ura, Nobuyuki, Shimamoto, Kazuaki, Sampaio, Claudio A.M
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.1999; Elsevier
• Subjects: Alligators and Crocodiles - blood; Amino Acid Sequence; Amino terminal sequence; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Blood Proteins - chemistry; Caiman crocodilus yacare; Enzymes and enzyme inhibitors; Freshwater; Fundamental and applied biological sciences. Psychology; Hydrolases; Kazal inhibitor; Molecular Sequence Data; Reptile; Sequence Homology, Amino Acid; Serine protease inhibitor; serine proteinase inhibitors; Serine Proteinase Inhibitors - blood; Serine Proteinase Inhibitors - chemistry; Trypsin Inhibitor, Kazal Pancreatic - blood; BK, bradykinin (Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg); Kazal inhibitor; MeO, methyl ester; Amino terminal sequence; EDTA, ethylenediamine tetraacetic acid; Serine protease inhibitor; SDS-PAGE, polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate; HPLC, high performance liquid chromatography; Suc, succinyl; Tris, Tris-(hydroxymethyl)-aminometane (The classical designation for l-amino acids is omitted; only d-isomers are indicated.); Reptile; Ac, acetyl; CcTI, Caiman crocodilus yacare plasma trypsin inhibitor; pNan, p-nitroanilide; Bz, Benzoyl; Pip, pipecolinic acid; SBTI, soybean trypsin inhibitor; TFA, trifluoroacetic acid; Blood coagulation; Purification; Serine endopeptidases; Enzyme; Enzyme inhibitor; Blood; Peptidases; Vertebrata; Substrate specificity; Hydrolases; Reptilia; Aminoacid sequence
• ISSN: 0162-3109
• DOI: 10.1016/S0162-3109(99)00145-9

Blood serine protease inhibitors are becoming better understood and increasingly applied in blood clotting, cancer and other diseases. Reptiles are suitable models for blood coagulation and related processes, moreover, caiman is a good comparative model of a non-poisonous reptile. Recently, we reported the purification of a kininogen, the presence of proteases involved in blood clotting, and a serine protease inhibitor in Caiman crocodilus yacare plasma. In this paper, we described the partial sequence of an inhibitor (CcTI). The inhibitor is an 80-kDa protein, and it inactivates trypsin and chymotrypsin the hydrolysis of specific chromogenic substrates and in the degradation of gelatin. The inhibitor is member of Kazal-type inhibitor family and consists of several domains, its putative reactive site is Arg-His.