Expression of a cDNA clone corresponding to the long open reading frame (X BL-I) of the bovine leukemia virus
Nucleotide sequence analysis of a cDNA clone corresponding to the X BL-I open reading frame of bovine leukemia virus (BLV) revealed that the AUG initiation codon was located 44 bases downstream from that of the env gene and was part of the p34 x mRNA splice donor ... ATGG/G TAA at the end of the pol...
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Published in | Virology (New York, N.Y.) Vol. 160; no. 1; pp. 55 - 59 |
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Main Authors | , , , , |
Format | Journal Article Web Resource |
Language | English |
Published |
United States
Elsevier Inc
01.09.1987
Academic Press |
Subjects | |
Online Access | Get full text |
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Summary: | Nucleotide sequence analysis of a cDNA clone corresponding to the X
BL-I open reading frame of bovine leukemia virus (BLV) revealed that the AUG initiation codon was located 44 bases downstream from that of the
env gene and was part of the p34
x mRNA splice donor ...
ATGG/G
TAA at the end of the
pol gene sequence. RNA from this clone was synthesized
in vitro by the SP6 RNA polymerase and translated into a 34,000 mol wt protein in rabbit reticulocyte lysates. The protein (p34
x) is recognized in Western blots by most sera of BLV-infected sheep and tumor-bearing cattle, by an anti-synthetic peptide rabbit serum, and by the serum of a rabbit immunized by X
BL-I RNA programmed reticulocyte lysates. Both sera react with a 34,000 mol wt protein present in nuclei of BLV-infected cells. |
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Bibliography: | L73 880286588 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 scopus-id:2-s2.0-0023413497 |
ISSN: | 0042-6822 1096-0341 1096-0341 |
DOI: | 10.1016/0042-6822(87)90043-2 |