Determination of the antidepressant fluoxetine in human plasma by LC with UV detection

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 23; no. 6; pp. 989 - 996
• Main Authors: Maya, Manuela T., Domingos, Constantino R., Guerreiro, M.Teresa, Morais, José A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.11.2000; Elsevier Science
• Subjects: Analysis; Antidepressive Agents, Second-Generation - blood; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; fluoxetine; Fluoxetine (FLX); Fluoxetine - blood; General pharmacology; High-performance liquid chromatography (HPLC); Humans; Linear Models; Medical sciences; Pharmacology. Drug treatments; Quantitative analysis; Ultraviolet Rays; High-performance liquid chromatography (HPLC); Fluoxetine (FLX); Quantitative analysis; Human; Validation; Fluoxetine; Psychotropic; Single dose; Oral administration; HPLC chromatography; Bioavailability; Normal; Blood plasma; Ultraviolet detector; Antidepressant agent; Pharmacokinetics
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(00)00367-8

A selective and sensitive isocratic high-performance liquid chromatographic (HPLC) method was developed for the quantitative analysis of low concentrations of fluoxetine (FLX) in human plasma, with ultra-violet detection at 226 nm. A reversed-phase column, LiChrospher® 60 RP-Select B (125×3 mm i.d., 5 μm) (Merck), was used to resolve FLX and diazepam (DZP) (internal standard) from endogenous matrix interferences. FLX was isolated from plasma by liquid-liquid extraction. Two identical HPLC systems were used, both validated under the same study conditions. Each chromatographic separation was completed in 30 min and the results showed a mean relative recovery of 101 and 99.3% and an overall precision (RSD%) of 4.78 and 6.09 for each HPLC system. The standard curve was linear for FLX concentrations over the range of 5.00–50.0 ng ml −1 ( R=0.997 and 0.998).The limit of quantitation of FLX was 5 ng ml −1 for both HPLC systems. The method described was applied to the analysis of plasma samples obtained from healthy subjects treated with one single oral dose of 40 mg of fluoxetine.