Engineered CaM2 modulates nuclear calcium oscillation and enhances legume root nodule symbiosis
SignificanceOscillations in intracellular calcium concentration play an essential role in the regulation of multiple cellular processes. In plants capable of root endosymbiosis with nitrogen-fixing bacteria and/or arbuscular mycorrhizal fungi, nuclear localized calcium oscillations are essential to...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 119; no. 13; p. e2200099119 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
29.03.2022
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Subjects | |
Online Access | Get full text |
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Summary: | SignificanceOscillations in intracellular calcium concentration play an essential role in the regulation of multiple cellular processes. In plants capable of root endosymbiosis with nitrogen-fixing bacteria and/or arbuscular mycorrhizal fungi, nuclear localized calcium oscillations are essential to transduce the microbial signal. Although the ion channels required to generate the nuclear localized calcium oscillations have been identified, their mechanisms of regulation are unknown. Here, we combined proteomics and engineering approaches to demonstrate that the calcium-bound form of the calmodulin 2 (CaM2) associates with CYCLIC NUCLEOTIDE GATED CHANNEL 15 (CNGC15s), closing the channels and providing the negative feedback to sustain the oscillatory mechanism. We further unraveled that the engineered CaM2 accelerates early endosymbioses and enhanced root nodule symbiosis but not arbuscular mycorrhization. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by Sharon Long, Stanford University, Stanford, CA; received January 6, 2022; accepted February 22, 2022 Author contributions: M.C. designed research; P.d.C, N.M.C., R.H., P.D., L.E.G., C.M., and A.H.C.L. performed research; P.d.C., N.M.C., P.D., and M.C. analyzed data; and M.C. wrote the paper. 3Present address: The Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom. 2Present address: Laboratory of Molecular Biology, 6708 PB, Wageningen, The Netherlands. 1P.d.C. and N.M.C contributed equally to this work. |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.2200099119 |