Investigation of meperidine and its metabolites in urine of an addict by gas chromatography—flame ionization detection and gas chromatography—mass spectrometry

A gas chromatographic method with flame ionization detection (FID) for the quantitative analysis of meperidine and mass spectrometry (MS) for the qualitative analyses of meperidine and its metabolites in urine was established. Meperidine, normeperidine and acetyl normeperidine in urine were extracte...

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Bibliographic Details
Published inJournal of chromatography. B, Biomedical applications Vol. 658; no. 2; pp. 375 - 379
Main Authors Feng, Liu, Xuying, Hu, Yi, Luo
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 19.08.1994
Elsevier
Subjects
Analysis
Biological and medical sciences
Biotransformation
Gas Chromatography-Mass Spectrometry
General pharmacology
Humans
Medical sciences
Meperidine - pharmacokinetics
Meperidine - urine
Opioid-Related Disorders - urine
Pharmacology. Drug treatments
Human
Urine
Metabolite
Opiates
Flame ionization detector
Narcotic analgesic
Intramuscular administration
Gas chromatography
Analysis method
Qualitative analysis
Pharmacokinetics
Mass spectrometry
Quantitative analysis
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Summary:A gas chromatographic method with flame ionization detection (FID) for the quantitative analysis of meperidine and mass spectrometry (MS) for the qualitative analyses of meperidine and its metabolites in urine was established. Meperidine, normeperidine and acetyl normeperidine in urine were extracted with ether. Free and conjugated meperidinic and normeperidinic acids in urine, which are hydrophillic, were hydrolyzed by acid, esterified with methanol and derivatized with acetic anhydride. Meperidine and its four metabolites were identified by GC—MS. Meperidine was measured by GC—FID during 72 h after intramuscular administration of meperidine to an addict.
ISSN:0378-4347
1572-6495
DOI:10.1016/0378-4347(94)00240-1