A comprehensive protein expression profile of extracellular matrix biomaterial derived from porcine urinary bladder

• Published in: Regenerative medicine Vol. 7; no. 2; pp. 159 - 166
• Main Authors: Marçal, Helder, Ahmed, Tania, Badylak, Stephen F, Tottey, Stephen, Foster, L John R
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01.03.2012
• Subjects: Animals; Biocompatible Materials - metabolism; biomaterial; Biomedical materials; Bladder; ECM; Extracellular matrix; Extracellular Matrix - metabolism; Extracellular Matrix Proteins - classification; Extracellular Matrix Proteins - metabolism; Health aspects; Male; Physiological aspects; Protein Transport; proteomics; Proteomics - methods; scaffold; Subcellular Fractions - metabolism; Sus scrofa - metabolism; tissue engineering; tissue regeneration; UBM; Urinary Bladder - metabolism; urinary bladder matrix; Australia; United States
• ISSN: 1746-0751; 1746-076X
• DOI: 10.2217/rme.12.6

To generate a comprehensive profile of the protein composition of xenogeneic biomaterial, derived from porcine urinary bladder matrix (UBM). Tunica layers and muscularis mucosa were removed from bladders using mechanical delamination. UBM was prepared using a solution of peracetic acid in ethanol, lyophilized then milled into powder. UBM biomaterial was subjected to tryptic digests and components separated using high-performance liquid chromatography with an ion trap mass spectrometer and identified through databases. A repertoire of 129 proteins with neurotrophic, antiangiogenic and tumor-suppressive activities and those associated with tissue remodeling and wound repair were identified. This study provides the first insight into the complex nature of the UBM and how its application may be tailored for specific applications in regenerative medicine. We propose that the UBM be further investigated for reconstructive and regenerative remodeling of cardiac and dermal tissues, as well as peripheral nerves.