Identification of the Arabidopsis thaliana Flavonoid 3'-Hydroxylase Gene and Functional Expression of the Encoded P450 Enzyme
The phenylpropanoid pathway results in the synthesis of thousands of compounds, including flavonoids like flavonols, anthocyanidins and tannins. In Arabidopsis thaliana, the lack of tannins in the seed coat (testa) causes the transparent testa (tt) phenotype. In the present study, we identified the...
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Published in | Biological chemistry Vol. 381; no. 8; pp. 749 - 753 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
Walter de Gruyter
01.08.2000
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Subjects | |
Online Access | Get full text |
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Summary: | The phenylpropanoid pathway results in the synthesis of thousands of compounds, including flavonoids like flavonols, anthocyanidins and tannins. In Arabidopsis thaliana, the lack of tannins in the seed coat (testa) causes the transparent testa (tt) phenotype. In the present study, we identified the gene responsible for the tt7 mutation. We show that TT7 encodes the enzyme flavonoid 3′-hydroxylase (F3′H), and demonstrate that this P450-dependent monooxygenase has F3′H activity. The availability of the AtF3′H gene and promoter sequence will allow us to study the coregulation of a complete set of flavonol and anthocyanidin biosynthesis genes in A. thaliana, and makes in vitro synthesis of hydroxylated flavonoids more feasible. |
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Bibliography: | bc.2000.095.pdf istex:55C654C7F09107908693B230396F222A5B798B74 ark:/67375/QT4-8C1W6PXM-S ArticleID:bchm.381.8.749 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1431-6730 |
DOI: | 10.1515/BC.2000.095 |