Appearance of Drug Resistance-Associated Mutations in Human Immunodeficiency Virus Type 1 CRF01_AE Integrase Derived from Drug-Naive Thai Patients

• Published in: AIDS research and human retroviruses Vol. 26; no. 12; p. 1341
• Main Authors: Isarangkura-Na-Ayuthaya, Panasda, Kaewnoo, Wiyada, Auwanit, Wattana, de Silva, U Chandimal, Ikuta, Kazuyoshi, Sawanpanyalert, Pathom, Kameoka, Masanori
• Format: Journal Article
• Language: English
• Published: United States 01.12.2010
• Subjects: Anti-HIV Agents - pharmacology; Drug Resistance, Viral; HIV Infections - virology; HIV Integrase - genetics; HIV-1 - drug effects; HIV-1 - genetics; HIV-1 - isolation & purification; Humans; Molecular Sequence Data; Mutation, Missense; Plasma - virology; RNA, Viral - genetics; Sequence Analysis, DNA; Thailand; Thailand
• ISSN: 1931-8405
• DOI: 10.1089/aid.2010.0140

CRF01_AE is a major subtype of human immunodeficiency virus type 1 (HIV-1) circulating in Southeast Asia, including Thailand. We performed genotypic studies on HIV-1 CRF01_AE integrase derived from plasma samples from drug-naive Thai patients. Direct sequencing of amplified CRF01_AE integrase genes revealed that although no primary mutations associated with drug resistance to integrase inhibitors were detected, at least one secondary mutation was found in 96% of samples. Our results indicate that the impact of these mutations on the baseline drug susceptibility of CRF01_AE viruses to integrase inhibitors may need to be addressed prior to the introduction of these drugs in Southeast Asian countries, including Thailand.