Protective effect of neurotrophic factors, neuropoietic cytokines and dibutyryl cyclic amp on hydrogen peroxide-induced cytotoxicity on PC12 cells: a possible link with the state of differentiation

• Published in: Neurochemistry international Vol. 33; no. 6; pp. 503 - 511
• Main Authors: Charon, I, Zuin-Kornmann, G, Bataille, S, Schorderet, M
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.12.1998; Elsevier
• Subjects: Animals; Biological and medical sciences; Bucladesine - pharmacology; Cell Differentiation; Cell physiology; Cell Survival - drug effects; Cytokines - pharmacology; Free Radicals; Fundamental and applied biological sciences. Psychology; Hydrogen Peroxide - antagonists & inhibitors; Hydrogen Peroxide - pharmacology; Molecular and cellular biology; Nerve Growth Factors - pharmacology; Neurons - cytology; Neurons - drug effects; Oxidative Stress; PC12 Cells; Progesterone - pharmacology; Rats; Responses to growth factors, tumor promotors, other factors; Dibutyryl cyclic AMP; Neurotrophic factor; Cell line; Neuron; Cytokine; Cytotoxicity; Differentiation; Hydrogen Peroxides; In vitro
• ISSN: 0197-0186; 1872-9754
• DOI: 10.1016/S0197-0186(98)00056-4

We present evidence that the survival of PC12 cells exposed to hydroxyl radicals generated by hydrogen peroxide applied for 30 min at 1 mM was effective when they were differentiated in response to Nerve Growth Factor (NGF) and/or other inducers of neurite outgrowth such as basic-fibroblast growth factor and dibutyryl cyclic AMP. The time- and dose-dependent differentiation triggered by NGF was (1) markedly increased by basic fibroblast growth factor, interleukin-6 or dibutyryl cyclic AMP; (2) diminished by leukemia inhibitory factor or ciliary neurotrophic factor; (3) not potentiated by insulin-like growth factor I or progesterone. The influence of these various factors and agents on PC12 cells was evaluated by the estimation of neurite outgrowth, whereas their possible protective effects were assessed by the measurement of cell survival. Our results would indicate that the factors and agents that induced differentiation were also able to protect the cells against an oxidative stress.