Vaccination with a Plasmodium chabaudi adami multivalent DNA vaccine cross-protects A/J mice against challenge with P. c. adami DK and virulent Plasmodium chabaudi chabaudi AS parasites

• Published in: International journal for parasitology Vol. 38; no. 7; pp. 819 - 827
• Main Authors: Scorza, T., Grubb, K., Cambos, M., Santamaria, C., Malu, D. Tshikudi, Spithill, T.W.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.2008; Elsevier Science
• Subjects: Animals; Antibody Specificity; Antigens, Protozoan - immunology; Biological and medical sciences; Cellular immune responses; Cross-protection; DNA vaccines; Female; Fundamental and applied biological sciences. Psychology; Genetic restriction; Interferon-gamma - blood; Life cycle. Host-agent relationship. Pathogenesis; Malaria; Malaria - immunology; Malaria - prevention & control; Mice; Mice, Inbred A; Multivalency; Opsonin Proteins; Opsonizing antibodies; Parasitemia; Plasmodium chabaudi; Plasmodium chabaudi - immunology; Plasmodium chabaudi - physiology; Plasmodium chabaudi chabaudi; Protozoa; Vaccination; Vaccines, DNA - administration & dosage; Virulence; Multivalency; Malaria; Genetic restriction; Cellular immune responses; Opsonizing antibodies; DNA vaccines; Cross-protection; Plasmodium chabaudi; Protozoal disease; Cross protection; Vaccination; Organism defense; Genetics; Protozoa; Apicomplexa; Immune response; Antibody; Rodentia; Parasite; Parasitosis; Genetic vaccine; Infection; Vertebrata; Mammalia; Mouse
• ISSN: 0020-7519; 1879-0135
• DOI: 10.1016/j.ijpara.2007.10.009

A current goal of malaria vaccine research is the development of vaccines that will cross-protect against multiple strains of malaria. In the present study, the breadth of cross-reactivity induced by a 30K multivalent DNA vaccine has been evaluated in susceptible A/J mice (H-2a) against infection with the Plasmodium chabaudi adami DK strain and a virulent parasite subspecies, Plasmodium chabaudi chabaudi AS. Immunized A/J mice were significantly protected against infection with both P. c. adami DK (31–40% reduction in cumulative parasitemia) and P. c. chabaudi AS parasites, where a 30–39% reduction in cumulative parasitemia as well as enhanced survival was observed. The 30K vaccine-induced specific IFN-γ production by splenocytes in response to native antigens from both P. c. chabaudi AS and P. c. adami DK. Specific antibodies reacting with surface antigens expressed on P. c. adami DS and P. c. chabaudi AS infected red blood cells, and with opsonizing properties, were detected. These results suggest that multivalent vaccines encoding conserved antigens can feasibly induce immune cross-reactivity that span Plasmodium strains and subspecies and can protect hosts of distinct major histocompatibility complex haplotypes.