DNA Vaccination with the Major Outer-Membrane Protein Gene Induces Acquired Immunity to Chlamydia trachomatis (Mouse Pneumonitis) Infection
The efficacy of DNA vaccination for prevention of Chlamydia trachomatis infection was studied using the murine model of pneumonia induced by the mouse pneumonitis (MoPn) isolate of C. trachomatis. Intramuscular DNA immunization with two chlamydial genes, one that encodes the major outer-membrane pro...
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Published in | The Journal of infectious diseases Vol. 176; no. 4; pp. 1035 - 1040 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Chicago, IL
The University of Chicago Press
01.10.1997
University of Chicago Press Oxford University Press |
Subjects | |
Online Access | Get full text |
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Summary: | The efficacy of DNA vaccination for prevention of Chlamydia trachomatis infection was studied using the murine model of pneumonia induced by the mouse pneumonitis (MoPn) isolate of C. trachomatis. Intramuscular DNA immunization with two chlamydial genes, one that encodes the major outer-membrane protein (MOMP) and one that encodes a cytoplasmic enzyme (cytosine triphosphate [CTP] synthetase) were tested. The MOMP DNA vaccine but not the CTP synthetase DNA vaccine generated significant delayed-type hypersensitivity and serum antibodies to MoPn elementary bodies and reduced the peak growth of MoPn by >100-fold following lung challenge infection. MOMP DNA immunization suggests a new approach to vaccine development for prevention of human chlamydial infection. |
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Bibliography: | Reprints or correspondence: Dr. Robert C. Brunham, Dept. of Medical Microbiology, University of Manitoba, Room 543, 730 William Ave., Winnipeg, Manitoba, R3E 0W3 Canada. istex:50F804BD8BF94B8CCA4F89F0F3A6F221DCF078AF ark:/67375/HXZ-48MLT60H-N ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-1899 1537-6613 |
DOI: | 10.1086/516545 |