Inhibition of the hyperalgesic activity of Bothrops jararaca venom by an antibothropic fraction isolated from opossum ( Didelphis marsupialis) serum

• Published in: Toxicon (Oxford) Vol. 38; no. 6; pp. 875 - 880
• Main Authors: Rocha, Surza L.G, Frutuoso, Valber S, Domont, Gilberto B, Martins, Marco A, Moussatché, Haity, Perales, Jonas
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.2000; Elsevier Science
• Subjects: Animal poisons toxicology. Antivenoms; Animals; Anti-Inflammatory Agents - therapeutic use; Anti-Inflammatory Agents, Non-Steroidal - therapeutic use; Antivenins - blood; Antivenins - therapeutic use; Azepines - therapeutic use; Biological and medical sciences; Bothrops; Bothrops jararaca; Bothrops jararaca venom; Crotalid Venoms - antagonists & inhibitors; Crotalid Venoms - toxicity; Dexamethasone - therapeutic use; Didelphis marsupialis; Didelphis marsupialis resistance; hyperalgesia; Hyperalgesia - chemically induced; Hyperalgesia - prevention & control; Hyperalgesic activity; Indomethacin - therapeutic use; Medical sciences; Natural immunity; Opossums - blood; Platelet Aggregation Inhibitors - therapeutic use; Toxicology; Triazoles - therapeutic use; Hyperalgesic activity; Natural immunity; Bothrops jararaca venom; Didelphis marsupialis resistance; Rat; Bothrops jararaca; Rodentia; Animal origin; Ophidia; Hyperalgesia; Didelphis marsupialis; Resistance; Vertebrata; Mammalia; Pain; Treatment; Animal; Venom; Marsupialia; Serum; Reptilia; Inhibition; Neutralization
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/S0041-0101(99)00190-7

The antibothropic fraction (ABF) already isolated from Didelphis marsupialis serum, inhibits the haemorrhagic, oedematogenic, myonecrotic and lethal activities of Bothrops jararaca venom (Bjv). The aim of this work was to verify the capability of ABF to inhibit the hyperalgesic activity of Bjv. Intraplantar injection of Bjv induced hyperalgesia in a time- and dose-dependent manner and ABF administered in situ concomitantly with Bjv or i.v. 30 min before venom injection reduced the induced hyperalgesia. This same effect was observed when ABF was intravenously injected at 5 and 15 min after Bjv. Our results show that ABF inhibits also the hyperalgesia induced by Bjv.