Voltage-dependent calcium channel subtypes controlling somatic substance P release in the peripheral nervous system

• Published in: Progress in neuro-psychopharmacology & biological psychiatry Vol. 23; no. 6; pp. 1103 - 1112
• Main Authors: Harding, Louise M., Beadle, David J., Isabel, Bermudez
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.08.1999; Elsevier
• Subjects: Animals; Biological and medical sciences; Calcium Channel Blockers - pharmacology; calcium channels; Calcium Channels - drug effects; Calcium Channels - physiology; Calcium Channels, L-Type - drug effects; Calcium Channels, N-Type - drug effects; Calcium Channels, P-Type - drug effects; Cell physiology; Electrophysiology; Female; Fundamental and applied biological sciences. Psychology; Ganglia, Spinal - cytology; Ganglia, Spinal - metabolism; Ganglia, Spinal - ultrastructure; Immunohistochemistry; In Vitro Techniques; Ion Channel Gating - drug effects; Ion Channel Gating - physiology; Male; Microscopy, Electron; Molecular and cellular biology; Neurons - drug effects; Neurons - metabolism; Neurons - ultrastructure; peptide release; Peripheral Nervous System - drug effects; Peripheral Nervous System - metabolism; Potassium - pharmacology; Rats; Rats, Sprague-Dawley; Secretion. Exocytosis; sensory neurones; Substance P; Substance P - metabolism; tris buffered saline; Substance P; calcium channels; small synaptic vesicles; dorsal root ganglia; large dense core vesicles; sensory neurones; voltage-dependent calcium channels; peptide release; goat serum; Peripheral nervous system; Rat; Potassium ion; Rodentia; Ionic channel; In vitro; Neurosecretion; Depolarization; Ganglion neuron; Vertebrata; Mammalia; Calcium ion
• ISSN: 0278-5846; 1878-4216
• DOI: 10.1016/S0278-5846(99)00049-4

1. 1. Isolated rat dorsal root ganglia (DRG) neurones support vesicular, non synaptic release of substance P in a depolarisation and Ca ++ dependent manner. 2. 2. In vivo this process may mediate cross-communication between DRG cells in some neuropathological conditions and is therefore a putative area for drug intervention. 3. 3. The authors investigated the voltage-dependent Ca ++ channel (VDCC) subtypes involved in somatic release of Substance P. Fresh (< 1 day) cultures of DRG neurones were incubated with high K + depolarising saline in the presence and absence of subtype selective VDCC blockers. Substance P released into the external media was collected and quantified using a radioimmunoassay. 4. 4. The results show that L-type and N-type, but not P-type, VDCCs play an important role in high K + evoked substance P release from rat DRG neurones.