Reversed-phase-liquid chromatography method for separation and quantification of gallic acid from hydroalcoholic extracts of Qualea grandiflora and Qualea parviflora

• Published in: Pharmacognosy Magazine Vol. 11; no. Suppl 2; pp. S316 - S321
• Main Authors: de Mesquita, Mariana L, Leão, Waleska F, Ferreira, Magda R A, de Paula, José E, Espindola, Laila S, Soares, Luiz A L
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01.10.2015; Sage Publications Ltd; Medknow Publications & Media Pvt Ltd
• Subjects: Acids; Antioxidants; Chromatography; Ecosystems; High performance liquid chromatography; Membrane filters; Methods; Original; Pharmacological research; Plant extracts; Quality control; Solvents; Surveillance; Ulcers; Brazil; Cerrado Biome; Qualea grandiflora; Gallic acid; high-performance liquid chromatography with diode array detection; Qualea parviflora; validation
• ISSN: 0973-1296; 0976-4062
• DOI: 10.4103/0973-1296.166062

Qualea parviflora and Qualea grandiflora (Vochysiaceae), commonly known in Brazil as "pau-terra" and "pau-terrinha," respectively, have been widely used in the treatment of ulcer and gastritis. These therapeutic effects are attributed to various compounds present in the plants, including phenolic compounds such as gallic acid, due to their important antioxidant activity. The aim of the present study was to validate a high performance liquid chromatography with diode array detection (HPLC-DAD) method for the quantitative determination of gallic acid in the stem bark of Q. parviflora and Q. grandiflora hydroalcoholic extracts. The chromatography analysis was successfully achieved on a Dionex column, Acclaim(®) 120 (250 mm × 4.60 mm, 5 µm) with a gradient elution of water and methanol at a flow rate of 0.8 mL/min and ultraviolet detection at 280 nm. The validation data, including linearity, precision, specificity, accuracy and robustness of this method demonstrated good reliability and sensitivity. The method is able to quantify gallic acid in the stem bark of both species. What is more, the chromatographic peaks showed good resolution and there are also the advantages of easy sample preparation and a short time between each injection.