Immunomodulatory activity of novel adjuvant formulations based on Montanide ISA oil-based adjuvants and peptidoglycan monomer

• Published in: International immunopharmacology Vol. 8; no. 5; pp. 717 - 724
• Main Authors: Habjanec, Lidija, Halassy, Beata, Tomašić, Jelka
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2008; Elsevier
• Subjects: Adjuvants, Immunologic - chemistry; Adjuvants, Immunologic - pharmacology; Animals; Biological and medical sciences; Chemistry, Pharmaceutical; Cytokines - biosynthesis; Cytokines - metabolism; Enzyme-Linked Immunosorbent Assay; Freund's Adjuvant; Immunization; Immunoglobulin G - biosynthesis; Immunoglobulin G - genetics; Interferon-gamma - biosynthesis; Interleukin-4 - biosynthesis; Lymph Nodes - cytology; Lymph Nodes - drug effects; Mannitol - analogs & derivatives; Mannitol - chemistry; Mannitol - pharmacology; Medical sciences; Mice; Mice, Inbred CBA; Montanide ISA206; Montanide ISA720; Oleic Acids - chemistry; Oleic Acids - pharmacology; Ovalbumin - immunology; Peptidoglycan - chemistry; Peptidoglycan - pharmacology; Peptidoglycan monomer; Pharmacology. Drug treatments; Th1 Cells - drug effects; Th1 Cells - immunology; Th1/Th2; Th2 Cells - drug effects; Th2 Cells - immunology; Th1/Th2; Montanide ISA206; Peptidoglycan monomer; Montanide ISA720; Immunomodulation; Th1 lymphocyte; Biological activity; Immunomodulator; Peptidoglycan; Oil; Formulation; T-Lymphocyte; Adjuvant; Th2 lymphocyte; Monomer
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2008.01.017

The aim of the study was to directly compare the potential of Montanide ISA720 and ISA206 oil-based adjuvant formulations on the induction of Th1/Th2-type of immune response, and to compare their effect to Complete Freund's adjuvant (CFA), a well known Th1 inducer. IgG isotype profiles (IgG1/IgG2a ratios) and specific cytokine secretion (IFN-γ and IL-4) as specific markers of Th1/Th2-type of immune response were monitored in experimentally immunised mice using ovalbumin (OVA) as an antigen. Specifically, we wanted to evaluate whether the incorporation of immunostimulating peptidoglycan monomer (PGM) into two oil-based adjuvants (ISA720PGM and ISA206PGM) influences their capability on Th1/Th2-type of immune response switching. The experiments were carried out using two genetically different inbred strains of mice, i.e. CBA and NIH/OlaHsd mice, respectively. We found significant differences in immune responses related to the genetic background of the two mice strains used in the study. In both mice strains, ISA720 formulations had similar effect to the positive control, CFA, and induced the switch towards Th1-type of immune response specific for OVA. However, ISA206 formulations were less effective in inducing the switch towards Th1 in CBA mice, while in NIH/OlaHsd mice promoted the switch towards Th2-type of immune response.