Qualitative and quantitative simultaneous determination of six marker compounds in soshiho-tang by HPLC-DAD-ESI-MS

• Published in: Archives of pharmacal research Vol. 35; no. 10; pp. 1785 - 1791
• Main Authors: Yang, Hye Jin, Ma, Jin Yeul, Weon, Jin Bae, Lee, Bohyoung, Ma, Choong Je
• Format: Journal Article
• Language: English
• Published: Heidelberg Pharmaceutical Society of Korea 01.10.2012; 대한약학회
• Subjects: Chromatography, High Pressure Liquid - methods; Medicine; Medicine, Korean Traditional; Molecular Structure; Pharmacology/Toxicology; Pharmacy; Plant Extracts - analysis; Plant Extracts - chemistry; Plant Extracts - standards; Powders; Quality Control; Reference Standards; Reproducibility of Results; Research Article; Spectrometry, Mass, Electrospray Ionization - methods; 약학; Simultaneous determination; Method validation; HPLC-DAD-ESI-MS; Soshiho-tang
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-012-1011-0

Soshiho-tang, one of the herbal prescriptions, has been used in treatment of chronic liver disease. In this study, the efficient high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS) method was developed and validated for simultaneous determination of six marker compounds, namely homogentisic acid, baicalin, glycyrrhizin, saikosaponin A, 6-gingerol and ginsenoside Rg3 in Soshiho-tang. The analysis of six marker compounds was carried out using a C 18 column (SHISEIDO S-5 μm, 4.6 mm I.D. × 250 mm) and gradient elution with water − 0.1% trifluoroacetic acid and acetonitrile. The analytical method was validated for linearity, precision and accuracy. Calibration curve for six marker compounds showed good linear regression (r 2 > 0.9996). The limits of detection (LOD) and limits of quantification (LOQ) for analytes were ranged from 0.04 to 0.16 μg/mL and 0.12 to 0.47 μg/mL, respectively. The relative standard deviations (RSD%) of the precision test, intra- and inter-day, were less than 0.32% and 0.10%, respectively. All recoveries of the six marker compounds were ranged from 100.05% to 102.25% with RSD less than 0.63%. These results have shown that this developed method was efficient for the quality evaluation of Soshiho-tang.