Effect of L-carnitine on in vitro developmental rate, the zona pellucida and hatching of blastocysts and their cell numbers in mouse embryos

L-carnitine (LC) is an antioxidant with the ability to promote the growth in vitro embryo. The goal was to evaluate the effect of LC on some indicators of embryo development and blastocyst quality including zona pellucid (ZP) thickness, the hatching of blastocysts and their cell numbers. Mouse embry...

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Published inInternational journal of reproductive biomedicine (Yazd, Iran) Vol. 14; no. 10; pp. 649 - 656
Main Authors Khanmohammadi, Nasrin, Movahedin, Mansoureh, Safari, Manouchehr, Sameni, Hamid Reza, Yousefi, Behpour, Jafari, Behnaz, Zarbakhsh, Sam
Format Journal Article
LanguageEnglish
Published Iran Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility 01.10.2016
Research and Clinical Center for Infertility
Subjects
Original
L-carnitine
Zona pellucida
Blastocyst inner cell mass
Embryo culture
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Summary:L-carnitine (LC) is an antioxidant with the ability to promote the growth in vitro embryo. The goal was to evaluate the effect of LC on some indicators of embryo development and blastocyst quality including zona pellucid (ZP) thickness, the hatching of blastocysts and their cell numbers. Mouse embryos were randomly divided into five groups and incubated with different concentrations of LC (I; 0, II; 0.5, III; 1, IV; 2 and V; 4 mg/ml) from 2-cell to hatched blastocyst. The percentage of blastocysts and hatched blastocysts was calculated. Blastocysts ZP thickness was measured and the number of blastocyst cells was counted using Hoechst and propidium iodide (PI) staining. The results showed concentration of 0.5 mg/ml of LC had an antioxidant effect as in this group, the percentage of blastocysts and hatched blactocysts (p=0.01), the ZP thickness (p=0.00) and the number of blastocyst inner cell mass were significantly more favorable than the control group (p=0.03); and concentration of 4 mg/ml of LC had a toxic effect on embryo development and blastocyst quality (p=0.00). The results suggest that LC may increase the number of blastocyst cells, which probably helps to expand the blastocyst and thinning of the ZP thickness and, therefore, creating a successful hatching for implantation.
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ISSN:2476-4108
2476-3772
DOI:10.29252/ijrm.14.10.649