Booster immunization of HIV-1 negative volunteers with HGP-30 vaccine induces protection against HIV-1 virus challenge in SCID mice

• Published in: Vaccine Vol. 17; no. 1; pp. 64 - 71
• Main Authors: Sarin, P.S, Talmadge, J.E, Heseltine, P, Murcar, N, Gendelman, H.E, Coleman, R, Kelsey, L, Beckner, S, Winship, D, Kahn, J
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1999; Elsevier
• Subjects: Adult; AIDS Vaccines - immunology; AIDS Vaccines - therapeutic use; Animals; Biological and medical sciences; Cell Division - physiology; Enzyme-Linked Immunosorbent Assay; Female; Fundamental and applied biological sciences. Psychology; gag Gene Products, Human Immunodeficiency Virus; HGP-30; HIV Antibodies - blood; HIV Antigens - immunology; HIV Infections - prevention & control; HIV Seronegativity - immunology; HIV-1 - immunology; HIV-1 vaccine; Human immunodeficiency virus 1; Human viral diseases; Humans; Immunization, Secondary; Infectious diseases; Leukocytes, Mononuclear - immunology; Male; Medical sciences; Mice; Mice, SCID; Microbiology; Middle Aged; Peptides - immunology; SCID mice; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Virology; SCID mice; HIV-1 vaccine; HGP-30; Human; Immunoprotection; Immune response; Peptides; HIV-1 virus; Booster vaccination; Rodentia; Retroviridae; Cellular immunity; Vaccine; Lentivirus; Virus; Vertebrata; Mammalia; Immunogenicity; Mouse; Seronegativity; Human immunodeficiency virus; Severe; Combined immune deficiency
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(98)00119-4

Eleven HIV-1 seronegative subjects previously injected with an HIV-1 p17 synthetic peptide vaccine (HGP-30) were given two booster immunizations to evaluate memory cell responses and the ability to boost cellular and humoral immune responses. Five of 11 subjects showed a significant increase in their antibody titres to HGP-30 or p17 and 6/11 had T-cell proliferation responses to either HGP-30 or p17. HIV-1 virus challenge studies in SCID mice demonstrated that 39 of 50 mice (78%) receiving PBMC from 5 of the HGP-30 immunized subjects were protected from infection with a different strain of HIV-1 compared to 4 of 30 mice (13%) that received PBMC from 3 non-immunized subjects ( p<0.001). These studies show that booster immunizations with HGP-30 vaccine are safe and non-toxic and induce protective cell mediated immune responses.