Effects of kisspeptin-10 on progesterone secretion in cultured chicken ovarian granulosa cells from preovulatory (F1–F3) follicles

• Published in: Peptides (New York, N.Y. : 1980) Vol. 32; no. 10; pp. 2091 - 2097
• Main Authors: Xiao, Yunqi, Ni, Yingdong, Huang, Yanbing, Wu, Jing, Grossmann, Roland, Zhao, Ruqian
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.10.2011; Elsevier
• Subjects: 3-Hydroxysteroid Dehydrogenases - genetics; 3-Hydroxysteroid Dehydrogenases - metabolism; 3β-HSD; Animals; antibodies; Biological and medical sciences; Cells, Cultured; Chicken granulosa cells; Chickens; Cholesterol Side-Chain Cleavage Enzyme - genetics; Cholesterol Side-Chain Cleavage Enzyme - metabolism; cytochrome P-450; Female; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression - drug effects; gene expression regulation; granulosa cells; Granulosa Cells - cytology; Granulosa Cells - drug effects; Granulosa Cells - physiology; hens; immunocytochemistry; Kisspeptin-10; Kisspeptins - pharmacology; Ovarian Follicle - cytology; Ovarian Follicle - physiology; P450scc; Phosphoproteins - genetics; Phosphoproteins - metabolism; Progesterone; Progesterone - secretion; protein content; quantitative polymerase chain reaction; regulatory proteins; secretion; StAR; transcription (genetics); Vertebrates: endocrinology; viability; Chicken granulosa cells; Progesterone; P450scc; 3β-HSD; StAR; Kisspeptin-10; Secretion; Ovarian hormone; Progestagen; Kisspeptin; Ovary; Vertebrata; Animal; Female genital system; Chicken; Granulosa; Aves; Sex steroid hormone; Ovarian follicle
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2011.09.001

► Kisspeptin immunoreactive substance is expressed in chicken ovarian granulosa cells. ► Kisspeptin-10 (Kp-10) stimulates progesterone secretion in these cells from preovulatory (F1–F3) follicles. ► Stimulated progesterone secretion parallels upregulation of StAR, P450scc and 3β-HSD mRNA expression. The effect of kisspeptin-10 (Kp-10) on the secretion of progesterone (P4) was investigated in cultured granulosa cells from F1 to F3 follicles of hens. The results showed that granulosa cells were stained with clear signals for kisspeptin using immunocytochemistry with the specific antibody against Kp-10. Among 10, 100 and 1000nM concentrations tested, 100nM Kp-10 treated for 24h significantly increased P4 secretion in granulosa cells from F1 to F3 follicles. After 24h and 48h of treatment, 100nM Kp-10 showed a significant increase in P4 secretion, while after 72h of treatment P4 secretion was markedly decreased by Kp-10 compared to the control group (P<0.05). F1 and F2/3 cells treated with 100nM Kp-10 for 24h showed significantly increased viability (P<0.05) and which was in parallel to a marked increase in P4 secretion (P<0.01). Real-time PCR results showed that the gene expression of the steroidogenic acute regulatory protein (StAR), cytochrome P450 side-chain cleavage (P450scc) and the enzyme 3β-hydroxysteroid dehydrogenase (3β-HSD) in F1 and F2/3 granulosa cells was significantly up-regulated by 24h–100nM Kp-10 treatment (P<0.05 versus P<0.01, respectively). However, there was no significant difference in StAR, P450scc and 3β-HSD protein content between control and the Kp-10 treated group (P>0.05). These results indicate that Kp-10 stimulates P4 secretion in cultured chicken granulosa cells, which was associated with an up-regulation in StAR, P450scc and 3β-HSD gene transcription.