Effects of granulocyte colony-stimulating factor on repair of injured canine arteries

• Published in: Chinese medical journal Vol. 121; no. 2; pp. 143 - 146
• Main Authors: Mei, Que-lin, Yang, Jian-yong, Li, Yan-hao, Chen, Zai-zhong, Yu, Hong-jian, Liu, Peng-cheng
• Format: Journal Article
• Language: English
• Published: China Department of Interventional Radiology,First Affiliated Hospital,Sun Yat-Sen University,Guangzhou,Guangdong 510080,China%Department of Interventional Radiology,The First Affiliated Hospital,Sun Yat-Sen University,Guangzhou,Guangdong 510080,China%Department of Interventional Radiology,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong 510515,China%Department of Radiology,Peking University Shenzhen Hospital,Shenzhen Medical Centre,Peking University-Hong Kong University of Sciences and Technology,Shenzhen,Guangdong 518036,China 20.01.2008; Department of Radiology,Peking University Shenzhen Hospital,Shenzhen Medical Centre,Peking University-Hong Kong University of Sciences and Technology,Shenzhen,Guangdong 518036,China
• Subjects: Animals; Dogs; Granulocyte Colony-Stimulating Factor - pharmacology; Hematopoietic Stem Cell Mobilization; Hyperplasia; Male; Recombinant Proteins; Renal Artery - injuries; Renal Artery - pathology; Tunica Intima - pathology; 粒细胞; 细胞活素类; 血管成形术; restenosis; cytokines; angioplasty
• ISSN: 0366-6999; 2542-5641
• DOI: 10.1097/00029330-200801020-00010

Background Endothelial progenitor cells （EPCs） derived from bone marrow may differentiate into endothelial cells and participate in endothelial repair. These cells can be mobilized into peripheral blood by cytokines, including granulocyte colony-stimulating factor （G-CSF）. In the present study, we investigated the effects of G-CSF on neointimal formation and restenosis in a canine model of arterial balloon injury. Methods Sixteen male beagle dogs were injected subcutaneously with 20 μg·kg^-1·d^-1 recombinant human G-CSF （n=8） or normal saline （n=8） for 1 week. On the fifth day of treatment, the dogs underwent renal arterial angioplasty. At 8 weeks after arterial balloon injury, angiographic observations were made and injured arteries were processed for morphometric analysis of neointimal formation. Results Peripheral white blood cell counts were increased by 3.34-fold compared to baseline on the fifth day of administration of G-CSF. Angiographies revealed that one stenosis had occurred among the eight injured renal arteries from dogs treated with G-CSF, whereas all injured renal arteries from dogs treated with normal saline remained patent. The mean extent of stenosis among injured arteries was 18.3%±17.9% in the G-CSF treated group compared to 12.5%±7.6% in the saline treated control group （P=0.10）. G-CSF treatment slightly increased neointimal thickness （0.42±0.15 mm vs 0.25±0.06 mm, P=-0.08） with an intima to media ratio of 0.83±0.49 vs 0.54±0.18 （P=0.11）. Conclusions G-CSF treatment does not attenuate neointimal hyperplasia and restenosis formation in a canine model of renal arterial injury, suggesting that the therapeutic strategy for preventing restenosis by stem cell mobilization should be investigated further.