Cloning of sarco-endoplasmic reticulum Ca²⁺-ATPase (SERCA) from Caribbean spiny lobster Panulirus argus

• Published in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Vol. 179; no. 2; pp. 205 - 214
• Main Authors: Mandal, A, Arunachalam, S. C, Meleshkevitch, E. A, Mandal, P. K, Boudko, D. Y, Ahearn, G. A
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.02.2009; Springer-Verlag; Springer Nature B.V
• Subjects: Amino acids; Animal Physiology; Animals; Base Sequence; Biochemistry; Bioinformatics; Biological Transport, Active - physiology; Biology; Biomedical and Life Sciences; Biomedicine; Calcium; Calcium - metabolism; Cloning; Cloning, Molecular; Cluster Analysis; Computational Biology; Crustaceans; DNA Primers - genetics; DNA, Complementary - genetics; Economic importance; Endoplasmic reticulum; Endoplasmic Reticulum - enzymology; Human Physiology; In Situ Hybridization; Laboratories; Life Sciences; Molecular Sequence Data; Original Paper; Palinuridae - enzymology; Palinuridae - genetics; Phylogeny; Polymerase chain reaction; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Sarcoplasmic Reticulum Calcium-Transporting ATPases - genetics; Sequence Analysis, DNA; Shellfish; Zoology; United States > US; Polymerase chain reaction; Real time PCR; Sequencing; Cloning; Rapid amplification of cDNA ends; Spiny lobster
• ISSN: 0174-1578; 1432-136X
• DOI: 10.1007/s00360-008-0303-7

We have previously reported on calcium transport mechanisms in American lobster, Homarus americanus, using ⁴⁵Ca²⁺ coupled with vesicle preparations of hepatopancreatic endoplasmic reticulum. The active transport of calcium across membranes bordering calcium-sequestering stores such as sarcoplasmic or endoplasmic reticulum is catalyzed by membrane-spanning proteins, the sarco-endoplasmic Ca²⁺-ATPases (SERCAs). In the study described here we used advanced bioinformatics and molecular techniques to clone SERCA from the economically important Caribbean spiny lobster, Panulirus argus. We report the complete cloning of a full-length SERCA from P. argus antenna cDNA (GenBank accession number AY702617). This cDNA has a 1020-amino acid residue open reading frame which is 90% identical to published sequences of other crustacean SERCA proteins. Our data support the hypothesis that one crustacean and three vertebrate genes controlling calcium transport were derived from a common ancestral gene.