A recombinant fowlpox virus expressing the envelope antigen of subgroup A avian leukosis/sarcoma virus

A recombinant fowlpox virus (FPV) was constructed by inserting cloned sequences from Schmidt-Ruppin subgroup A avian sarcoma virus coding for the viral envelope (env) antigen into a nonessential region of FPV DNA downstream from a synthetic promoter. Sera from chickens hyperimmunized with the recomb...

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Bibliographic Details
Published inAvian diseases Vol. 39; no. 3; p. 514
Main Authors Nazerian, K. (USDA, ARS, Avian Disease and Oncology Laboratory, East Lansing, MI.), Yanagida, N
Format Journal Article
LanguageEnglish
Published United States 01.07.1995
Subjects
Alpharetrovirus - genetics
Animals
ANTIGENE
ANTIGENOS
Antigens, Viral - genetics
Avian Leukosis - immunology
Avian Leukosis - prevention & control
AVIPOXVIRUS
Base Sequence
Cells, Cultured
Chickens
CONTROL DE ENFERMEDADES
CONTROLE DE MALADIES
Fowlpox - immunology
Fowlpox - prevention & control
Fowlpox virus - genetics
Fowlpox virus - immunology
GENE
GENES
Genetic Vectors
LEUCOSE AVIAIRE
LEUCOSIS AVIAR
Molecular Sequence Data
PODER PATOGENO
POLLO
POULET
POUVOIR PATHOGENE
SARCOMA
Sarcoma, Avian - immunology
Sarcoma, Avian - prevention & control
SARCOME
VACCIN SYNTHETIQUE
VACUNA SINTETICA
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
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Summary:A recombinant fowlpox virus (FPV) was constructed by inserting cloned sequences from Schmidt-Ruppin subgroup A avian sarcoma virus coding for the viral envelope (env) antigen into a nonessential region of FPV DNA downstream from a synthetic promoter. Sera from chickens hyperimmunized with the recombinant FPV neutralized the infectivity of the homologous subgroup A virus (RCASBP/AP) but only weakly neutralized the infectivity of Rous sarcoma virus, another subgroup A avian leukosis virus. Similarly, vaccination of 1-day-old chicks with this recombinant FPV protected against infection with RCASBP/AP virus but not against infection with another subgroup A Rous-associated virus (RAV-1). These results show that such a recombinant FPV can be used to protect chickens against avian leukosis virus and confirm previous observations that a type-specific antigenic variability existed within the subgroup A avian leukosis/sarcoma virus group
Bibliography:L70
L73
9600167
ISSN:0005-2086
1938-4351
DOI:10.2307/1591803