A Carbohydrate Beverage Reduces Monocytes Expressing TLR4 in Children with Overweight or Obesity

• Published in: The Journal of nutrition Vol. 150; no. 3; pp. 616 - 622
• Main Authors: Niemiro, Grace M, Chiarlitti, Nathan A, Khan, Naiman A, De Lisio, Michael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2020; Oxford University Press; American Institute of Nutrition
• Subjects: Adipose tissue; Adolescent; adolescents; Beverages; Blood; Blood Glucose - metabolism; Body composition; Body weight; Carbohydrates; Child; Children; Childrens health; Correlation analysis; Cross-Over Studies; Cytokines - metabolism; diet; Dietary Carbohydrates - administration & dosage; Double-Blind Method; Dual energy X-ray absorptiometry; Female; Glucose; Glucose metabolism; Humans; Inflammation; Inflammation Mediators - metabolism; inflammatory cytokines; innate immune system; Insulin; Insulin - blood; Male; Monocytes; Monocytes - immunology; Monocytes - metabolism; Obesity; Obesity - immunology; Overweight; Overweight - immunology; Populations; Proteins; TLR4 protein; Toll-Like Receptor 4 - metabolism; Toll-like receptors; Variance analysis; EGG; inflammation; adolescents; PE; inflammatory cytokines; diet; CHO; OW/OB; TLR4; ES; innate immune system; HW
• ISSN: 0022-3166; 1541-6100
• DOI: 10.1093/jn/nxz294

Childhood obesity is increasing, with about one-third of children overweight or obese. Obesity is characterized by a state of chronic low-grade inflammation that is related to cardiometabolic comorbidities. Inflammatory monocytes, which are classified into 3 different groups—classical, intermediate, and nonclassical monocytes, with Toll-like receptor 4 (TLR4+) expression indicating a proinflammatory state—underlie several obesity-associated morbidities. This study aimed to assess the responses of monocyte populations to beverages of differing macronutrient composition in children with healthy weight (HW) or overweight/obesity (OW/OB). Ten HW children (5th to 84.9th percentile; mean age 12.29±2.5 y) and 7 children with OW/OB (85th to 99.99th percentile; mean age 11.96±3.8 y) completed the study. Adiposity was determined via DXA. Using a double-blinded, randomized, crossover design, participants consumed either a high-carbohydrate (CHO; 210 kcal; 0g fat/56g carbohydrates/0g protein) or a whole-eggbased high-protein/fat (EGG; 210 kcal; 15g fat/0g carbohydrates/18g protein) beverage. Venous blood was collected at baseline and 2 h postprandially for evaluation of metabolic and inflammatory responses. Repeated measures ANOVA and Pearson correlations were conducted. Consuming the CHO beverage significantly reduced the primary outcome: TLR4+expression on classical monocytes in children with OW/OB only (25.60% decrease from baseline in OW/OB compared with 1.61% increase in HW). Children with OW/OB had significantly less percentages of TLR4+nonclassical monocytes than HW (47.66% lower after CHO). Insulin and glucose (secondary outcomes), were significantly higher after the CHO condition compared with baseline (230.61% and 9.93% increase, respectively). Changes in glucose were significantly and negatively related to changes in monocyte populations in the CHO condition. These data suggest that high-carbohydrate beverages alter monocyte populations in the blood in children with OW/OB, which is related to glucose metabolism. These findings have implications for nutritional recommendations in children with overweight/obesity. National Clinical Trial registry trial number: NCT03597542.