Nutrient digestibility, digesta volatile fatty acids, and intestinal bacterial profile in growing pigs fed a distillers dried grains with solubles containing diet supplemented with a multi-enzyme cocktail

• Published in: Animal feed science and technology Vol. 212; pp. 70 - 80
• Main Authors: Agyekum, A.K., Regassa, A., Kiarie, E., Nyachoti, C.M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.02.2016
• Subjects: Bacteria; Bacterial profile; Digestibility; Distillers dried grains with solubles; Enterobacteriaceae; Firmicutes; Lactobacillus; Multicarbohydrase; Pig; AA; Multicarbohydrase; CAID; CATTD; NSP; Distillers dried grains with solubles; Bacterial profile; CP; N; SID; Pig; VFA; NDF; CCS; DDGS; BW; MC; ME; EC; GE; PCR; SBM; Digestibility
• ISSN: 0377-8401; 1873-2216
• DOI: 10.1016/j.anifeedsci.2015.12.006

•We evaluated a multicarbohydrase enzyme (MC) effect on nutrient digestibility, intestinal VFA and bacterial profile and in pigs fed a DDGS diet.•The MC improved DM, starch, AA and energy digestibility, but not NDF and N digestibility.•The MC stimulated the growth of fiber-fermenting bacteria in the DDGS-fed pigs.•However, the MC had no influence on digesta total VFA concentration or NDF fermentation. This study investigated the effects of adding a multi-enzyme cocktail (MC) to a distillers dried grains with solubles (DDGS)-containing diet on energy and nutrient coefficients of apparent ileal (CAID) and total tract (CATTD) digestibility, digesta volatile fatty acids (VFA) concentration, and gut bacterial profile using ileal-cannulated barrows (n=9; 62.7±6.4 Kg initial body weight). Three isocaloric/isonitrogenous diets based on corn and soybean meal with 0 (control) or 30% DDGS (DDGS diet) and DDGS diet supplemented with MC (DDGS+MC) were used. The 3 diets were fed to pigs in a 2-period change over design to obtain 6 observations per diet. A casein–cornstarch-based diet was used in a separate period to determine basal endogenous N and AA losses to estimate standardized ileal digestibility (SID) of AA. All diets contained titanium dioxide as a digestibility marker. Ileal digesta and feces were used for VFA and bacterial profile determination, respectively. The CAID for DM, N, starch, and energy were greater (P<0.05) in pigs fed the control diet than in pigs fed the DDGS diet. Addition of MC to the DDGS diet improved (P<0.05) the CAID for DM, starch, and energy, but not N (P>0.10). The DDGS diet also decreased (P<0.05) the CAID and SID for AA and the MC improved (P<0.05) the CAID and SID of some of the AA. Further, the DDGS diet decreased (P<0.05) the CATTD for DM, N, and energy and tended to decrease (P<0.10) the CATTD for NDF, whereas the MC improved (P<0.05) the CATTD for DM and energy, but not (P>0.10) for N and NDF. However, diet had no effect (P>0.10) on hindgut nutrient disappearance. Diet did not influence (P>0.10) digesta pH and VFA concentrations except for isovalerate concentration, which was greatest (P<0.05) in the DDGS+MC-fed pigs. Bacteroides–Prevotella–Porphyromonas and Enterobacteriaceae abundance were greatest (P<0.05) in the feces of the DDGS+MC-fed pigs. Additionally, the abundance of the β-xylosidase gene, xynB, from the Bacteroidetes group increased (P<0.05) when the MC was added to the DDGS diet. However, the DDGS-fed pigs had the greatest abundance (P<0.05) of Firmicutes. The abundance of Lactobacillus spp. was not affected (P>0.10) by diet. In conclusion, adding MC to the DDGS diet improved the digestibility of DM, starch, most AA, but not NDF and N digestibility. The results show that addition of MC to the DDGS diet stimulated the growth of intestinal bacteria with xylanolytic and cellulolytic activities.