Determination of creatinine and purine derivatives in ruminants' urine by reversed-phase high-performance liquid chromatography

A procedure is described for the rapid and simultaneous determination of allantoin, creatinine, uric acid, hypoxanthine and xanthine in sheep urine. Separation was achieved on a Novapak C18 column under isocratic conditions. The mobile phase was potassium phosphate buffer (10 mM, pH 4.0). A flow-rat...

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Bibliographic Details
Published inJournal of chromatography Vol. 607; no. 2; p. 199
Main Authors Resines, J A, Arín, M J, Díez, M T
Format Journal Article
LanguageEnglish
Published Netherlands 28.08.1992
Subjects
Animals
Chromatography, High Pressure Liquid
Creatinine - urine
Indicators and Reagents
Purines - urine
Sheep
Spectrophotometry, Ultraviolet
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Summary:A procedure is described for the rapid and simultaneous determination of allantoin, creatinine, uric acid, hypoxanthine and xanthine in sheep urine. Separation was achieved on a Novapak C18 column under isocratic conditions. The mobile phase was potassium phosphate buffer (10 mM, pH 4.0). A flow-rate of 0.5 ml/min, detection at 218 nm and a column temperature of 25 degrees C were employed with a total analysis time of less than 15 min. Detection limits for allantoin, creatinine, uric acid, hypoxanthine and xanthine were 1.0, 0.5, 0.5, 0.5 and 0.2 micrograms/ml, respectively, at a signal-to-noise ratio of 3 in a 20-microliters injection volume of tenfold-diluted urine. This sensitivity permits the precise determination of these compounds in ruminants' urine.
DOI:10.1016/0021-9673(92)87075-J