Cell surface detection of HLA-E gene products with a specific monoclonal antibody

• Published in: Journal of reproductive immunology Vol. 43; no. 2; pp. 195 - 201
• Main Authors: Pacasova, Rita, Martinozzi, Silvia, Boulouis, Henri-Jean, Szpak, Yann, Ulbrecht, Matthias, Sigaux, François, Weiss, Elisabeth H, Pla, Marika
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.07.1999; Elsevier Science
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; Antigens; Biological and medical sciences; Cell Membrane - immunology; Cytometry analysis; Fundamental and applied biological sciences. Psychology; Fundamental immunology; H-2 Antigens - immunology; Histocompatibility antigens (hla, h-2 and other systems); Histocompatibility Antigens Class I - analysis; Histocompatibility Antigens Class I - immunology; histocompatibility locus HLA; HLA Antigens - analysis; HLA Antigens - immunology; HLA-E; HLA-E Antigens; Humans; Mice; Mice, Transgenic; Molecular immunology; Spleen; Spleen; Cytometry analysis; HLA-E; Human; Flow cytometry; Immunization; Rodentia; Transgenic animal; Monoclonal antibody; Gene expression; Vertebrata; Biological fixation; Mammalia; Mouse; Class I histocompatibility antigen
• ISSN: 0165-0378; 1872-7603
• DOI: 10.1016/S0165-0378(99)00033-9

An HLA-E-specific monoclonal antibody (mAb) was obtained by immunization of human β2-microglobulin transgenic mice (M-TGM) with spleen cells from double transgenic mice expressing HLA-E molecules (EM-TGM). This mAb, designated V16, specifically recognizes in flow cytometry analysis the HLA-E expressing mouse cells, whereas it does not bind to mouse cells expressing various HLA class I molecules (HLA-A2, -A3, -A11, -A26, A29, -B7, -B27, -Cw3, -Cw7, and HLA-G). V16 mAb binds efficiently to human EBV-infected B lymphocytes, PHA blasts and PBL, thus establishing the surface expression of HLA-E in vivo on these cells.