Amyloid β precursor protein-mRNA is expressed throughout cerebral vessel walls

• Published in: Brain research Vol. 828; no. 1; pp. 179 - 183
• Main Authors: Natté, Remco, de Boer, Willem I., Maat-Schieman, Marion L.C., Baelde, Hans J., Vinters, Harry V., Roos, Raymund A.C., van Duinen, Sjoerd G.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 15.05.1999; Amsterdam Elsevier; New York, NY
• Subjects: Aged; Aged, 80 and over; Alzheimer Disease - genetics; Alzheimer Disease - metabolism; Alzheimer's disease; Amyloid beta-Protein Precursor - genetics; Amyloid β precursor protein; Biological and medical sciences; Brain - blood supply; Brain - metabolism; Cerebral amyloid angiopathy; Cerebral Amyloid Angiopathy - genetics; Cerebral Amyloid Angiopathy - metabolism; Cerebral Angiography; Cerebral Arteries - chemistry; Cerebral Arteries - physiology; Cerebral Hemorrhage - genetics; Cerebral Hemorrhage - metabolism; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Gene Expression; HCHWA-D; Humans; In Situ Hybridization; Medical sciences; Middle Aged; Neurology; RNA, Messenger - analysis; Amyloid β precursor protein; Cerebral amyloid angiopathy; HCHWA-D; In situ hybridization; Alzheimer's disease; Human; Nervous system diseases; Alzheimer disease; Central nervous system; Gene expression; Amyloid precursor protein; Cerebral disorder; Cerebral hemorrhage; Central nervous system disease; Degenerative disease; Amyloidosis; Vascular wall; Brain (vertebrata)
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(99)01361-X

To determine the presence and distribution of cerebrovascular Aβ production we investigated amyloid β precursor protein (AβPP)-mRNA expression by RNA in situ hybridization in patients with hereditary cerebral hemorrhage with amyloidosis, Dutch type, Alzheimer disease and controls. In all subjects, AβPP-mRNA was expressed in endothelial cells, smooth muscle cells, adventitial cells and brain pericytes and/or perivascular cells. Meningeal cells also expressed AβPP-mRNA. AβPP was detected in endothelial cells, smooth muscle cells and adventitial cells. The demonstration of AβPP-mRNA at all vascular sites where amyloid formation can occur supports an important contribution of locally derived Aβ to cerebrovascular amyloidosis.