Effects on protein and mRNA expression levels of p53 induced by fluoride in human embryonic hepatocytes

• Published in: Toxicology letters Vol. 158; no. 2; pp. 158 - 163
• Main Authors: Wang, A.G., Chu, Q.L., He, W.H., Xia, T., Liu, J.L., Zhang, M., Nussler, A.K., Chen, X.M., Yang, K.D.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 14.08.2005; Amsterdam Elsevier Science
• Subjects: Biological and medical sciences; Blotting, Western; Cell Line; Cell Survival - drug effects; Dose-Response Relationship, Drug; Fluoride; Fluorides - toxicity; Formazans - metabolism; Gene Expression - drug effects; Hepatocytes - drug effects; Hepatocytes - metabolism; Human embryo hepatocytes; Humans; Medical sciences; p53 mRNA; p53 Protein; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - drug effects; RNA, Messenger - metabolism; Tetrazolium Salts - metabolism; Toxicology; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; p53 mRNA; Fluoride; p53 Protein; Human embryo hepatocytes; Human; Embryo; Digestive system; Liver; Fluorides; TP53 Gene; Messenger RNA; Hepatocyte; Tumor suppressor gene
• ISSN: 0378-4274; 1879-3169
• DOI: 10.1016/j.toxlet.2005.03.010

We investigated the effects of protein and mRNA expression levels on p53 induced by fluoride in human embryo hepatocyte L-02 cells. The protein and mRNA levels of p53 in L-02 cells were measured after in vitro cultured L-02 was exposed to sodium fluoride at different doses (40, 80, and 160 μg/ml) for 24 h. The results showed that the cell survival rate of L-02 cells in the high dose fluoride group was significantly lower than that of the control group. The protein expression levels of p53 in the middle and high dose fluoride group were significantly higher than in the control group and elevated with increasing fluoride concentration. The mRNA expression levels of p53 in the fluoride groups were markedly higher than in the control group. The mRNA expression level of p53 in the high dose fluoride group was however lower compared to the middle dose fluoride group, but similar to the low dose fluoride group. These finding suggest that fluoride can decrease the L-02 cells survival rate and induce protein and mRNA expressions of p53; however, there is no consistency between the protein expression level of p53 and the mRNA expression level.