A microRNA-related single nucleotide polymorphism of the Dicer gene is associated with risk of dermatomyositis

• Published in: European journal of inflammation Vol. 21
• Main Authors: Peng, Chenxing, Zhang, Jingjing, Zhang, Shasha, Zhang, Xiaoyun, Zhao, Yufei
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.04.2023; SAGE PUBLICATIONS, INC; SAGE Publishing
• Subjects: Apoptosis; Cell growth; Cytokines; Gene expression; Health risks; MicroRNAs; Polymorphism; SNPs; DM; MiRNAs; Cytokine
• ISSN: 1721-727X; 2058-7392
• DOI: 10.1177/1721727X231173526

Objectives: To evaluate the correlation of miRNA-related single nucleotide polymorphisms (miR-SNPs) with the risk of dermatomyositis (DM) development. Introduction: MicroRNAs (MiRNAs) are involved in a variety of activities such as cell differentiation, proliferation, apoptosis, tumorigenesis, and immunological response. MiR-SNPs alter the expression levels of miRNAs, leading to increased susceptibility to DM. Methods: We genotyped six miR-SNPs for miRNA processing machinery genes, including XPO5 (rs11077), RAN (rs14035), Dicer (rs3742330), TNRC6B (rs9623117), GEMIN3 (rs197412), and GEMIN4 (rs2740348), and two miR-SNPs for microRNA binding site, including SET8 (rs16917496), and KRT81 (rs3660), in a case-control study to assess the impact of these miR-SNPs on DM risk. Then we assessed cytokine expression and ROS levels in DM to determine the relationship between risk-related miR-SNPs and cytokines. Results: We discovered that Dicer’s (rs3742330) AA genotype had a decreased chance of developing DM than the AG + GG type (odds ratio, 0.527; 95% confidence interval: 0.281–0.987; p = 0.045). The subsequent analysis showed that the AA genotype carrier had greater levels of IL-4 (p = 0.034). Conclusion: The SNP of Dicer (rs3742330) maybe an attractive predictor of DM, moreover the cytokine of IL-4 may act as the factor that distinguishes SNP of Dicer (rs3742330) into AA and AG + GG.