Using Speckle Dynamics for Comparison of the Metabolic Activity of Different Cell Cultures

The dynamics of speckles in the image plane of a monolayer of cells cultivated on a glass substrate has been recorded. Cell cultures HEL-3, L-41, and Vero were selected as the objects of research. The digitized value of the radiation intensity I in one pixel and the parameter η characterizing the ch...

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Published inRadiophysics and quantum electronics Vol. 57; no. 8-9; pp. 584 - 588
Main Authors Vladimirov, A. P., Malygin, A. S., Mikhailova, Yu. A., Borodin, E. M., Bakharev, A. A., Poryvaeva, A. P.
Format Journal Article
LanguageEnglish
Published Boston Springer US 01.01.2015
Springer
Springer Nature B.V
Subjects
Astronomy
Astrophysics and Astroparticles
Biotechnology
Coefficients
Digitization
Dynamics
Hadrons
Heavy Ions
Lasers
Mathematical and Computational Physics
Monolayers
Nuclear Physics
Observations and Techniques
Optical Devices
Optics
Photonics
Physics
Physics and Astronomy
Pixels
Planes
Quantum electronics
Quantum Optics
Theoretical
Speckle Pattern
Correlation Time
Laser Unit
Radiation Intensity
Speckle Contrast
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Summary:The dynamics of speckles in the image plane of a monolayer of cells cultivated on a glass substrate has been recorded. Cell cultures HEL-3, L-41, and Vero were selected as the objects of research. The digitized value of the radiation intensity I in one pixel and the parameter η characterizing the change in the intensity distribution on an 10 × 10 pixel area was recorded for 24 hours. The multiple determinacy coefficient of three cell cultures, which was obtained from the time dependences of η, was equal to 0.94.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0033-8443
1573-9120
DOI:10.1007/s11141-015-9542-0