Characterization of Cupric Glutamate Extinguishing Mechanism of Alexandrium sp. LC3 with Two-dimensional Electrophoresis and MALDI-TOF MS

• Published in: Marine biotechnology (New York, N.Y.) Vol. 10; no. 5; pp. 527 - 537
• Main Authors: Li, Hao, Miao, Jinlai, Cui, Fengxia, Li, Guangyou
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.09.2008; Springer-Verlag; Springer Nature B.V
• Subjects: Alexandrium; Alexandrium sp. LC3; Algae; Algicides; Analytical methods; Animals; Biomedical and Life Sciences; Cell Membrane Permeability - drug effects; Cell membranes; Clay; Copper; Copper - pharmacology; Cupric glutamate; Denaturation; Dinoflagellida - drug effects; Dinoflagellida - metabolism; Disintegration; DNA-directed RNA polymerase; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; Engineering; Extinguish; Extinguishing; Freshwater & Marine Ecology; Gene Expression Regulation - drug effects; Glutamic Acid - pharmacology; Herbicides; Ionization; Ions; Laboratories; Lasers; Life Sciences; Malondialdehyde; Malondialdehyde - metabolism; Mass spectrometry; Mass spectroscopy; Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry; Membrane permeability; Microbiology; Organisms; Original Article; Permeability; Pesticides - pharmacology; Plasma membrane permeability; Prepolymers; Protein expression; Proteins; Proteomics; Ribulose-bisphosphate carboxylase; RNA polymerase; Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Studies; Sulfhydryl Compounds - metabolism; Two-dimensional electrophoresis; Water quality; Zoology; Two-dimensional electrophoresis; Plasma membrane permeability; Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry; sp. LC3; Cupric glutamate; Extinguish
• ISSN: 1436-2228; 1436-2236
• DOI: 10.1007/s10126-008-9091-z

Mechanisms by which cupric glutamate, a novel algicide, extinguishes Alexandrium sp. LC3 are shown in this study. We show that cupric glutamate not only stimulated the production of malonaldehyde (MDA) and dramatically promoted cell plasma membrane permeability (p < 0.01) but also remarkably reduced sulfhydryl (SH) group content (p < 0.01). Analysis of protein expression profiles by two-dimensional electrophoresis (2-DE) indicated that only 47 protein spots were detected in both control and cupric glutamate treated cells. Three reliable spots were identified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as ribulose-bisphosphate carboxylase large subunit precursor, RNA polymerase beta chain, and hypothetical protein, which can be well correlated with cupric glutamate stress. Based on above results, we hypothesize that the extinguishing mechanisms include (1) the cell membrane being damaged by cupric glutamate; (2) cupric glutamate probably induced denaturation and disintegration of intracellular protein, which led to inhibition of cell growth.