Recirculating peritoneal dialysis system using urease-fixed silk fibroin membrane filter with spherical carbonaceous adsorbent

• Published in: Materials Science & Engineering C Vol. 97; pp. 55 - 66
• Main Authors: Sultan, Md. Tipu, Moon, Bo Mi, Yang, Jae Won, Lee, Ok Joo, Kim, Soon Hee, Lee, Ji Seung, Lee, Young Jin, Seo, Ye Been, Kim, Do Yeon, Ajiteru, Olatunji, Sung, Gun Yong, Park, Chan Hum
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2019; Elsevier BV
• Subjects: Acute Kidney Injury - therapy; Adsorbents; Animals; beta 2-Microglobulin - isolation & purification; Creatinine; Dialysis; End-stage renal disease; Enzymes, Immobilized - chemistry; Equipment Design; Exchanging; Fibroins - chemistry; Filtration; Filtration - instrumentation; In vivo methods and tests; Kidney diseases; Kidney Failure, Chronic - therapy; Kidneys; Male; Materials science; Membrane filter; Membranes, Artificial; Microscopy, Electron, Scanning; Patients; Peritoneal dialysis; Peritoneal Dialysis, Continuous Ambulatory - instrumentation; Peritoneal Dialysis, Continuous Ambulatory - methods; Peritoneum; Phosphorus; Phosphorus - isolation & purification; Rats, Sprague-Dawley; Silk; Silk fibroin; Spectroscopy, Fourier Transform Infrared; Toxins; Toxins, Biological - chemistry; Urea; Ureas; Urease; Urease - chemistry; Uremic toxins; Uric acid; Wearable artificial kidney; β2 Microglobulin; Membrane filter; Uremic toxins; Silk fibroin; Wearable artificial kidney; Peritoneal dialysis
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2018.12.021

The chronic kidney disease (CKD) patients are undergoing continuous ambulatory peritoneal dialysis (CAPD). However, there are some constraints, the frequent exchange of the dialysate and limitation of outside activity, associated with CAPD remain to be solved. In this study, we designed the wearable artificial kidney (WAK) system for peritoneal dialysis (PD) using urease-immobilized silk fibroin (SF) membrane and polymer-based spherical carbonaceous adsorbent (PSCA). We evaluated this kit's removal abilities of uremic toxins such as urea, creatinine, uric acid, phosphorus, and β2-microglobulin from the dialysate of end-stage renal disease (ESRD) patients in vitro. The uremic toxins including urea, creatinine, uric acid, and phosphorus were removed about 99% by immobilized SF membrane and PSCA filter after 24 h treatment. However, only 50% of β2-microglobulin was removed by this filtering system after 24 h treatment. In vivo study result shows that our filtering system has more uremic toxins removal efficiency than exchanged dialysate at every 6 h. We suggest that recirculating PD system using urease-immobilized SF membrane with PSCA could be more efficient than traditional dialysate exchange system for a WAK for PD. •Recirculating peritoneal dialysis kit constructed with urease-fixed SF membrane and PSCA•In vitro and in vivo evaluation of the uremic toxins removal efficiency of the filtering kit.•Comparable uremic toxins removal capacity of the filtering kit to the conventional peritoneal dialysis system