RFLP mapping of a Hordeum bulbosum gene highly expressed in pistils and its relationship to homoeologous loci in other Gramineae species

• Published in: Theoretical and applied genetics Vol. 105; no. 2-3; pp. 271 - 276
• Main Authors: GUDU, S, LAURIE, D. A, KASHA, K. J, XIA, J. J, SNAPE, J. W
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.08.2002; Berlin Springer Nature B.V
• Subjects: Barley; Biological and medical sciences; Cereals; Chromosomes; Classical genetics, quantitative genetics, hybrids; Cloning; Flowers & plants; Fundamental and applied biological sciences. Psychology; Gene expression; Genes; Genetic aspects; Genetics of eukaryotes. Biological and molecular evolution; Goat grass; Grasses; Physiological aspects; Plant genetics; Pteridophyta, spermatophyta; Research parks; Vegetals; Canada; United Kingdom; Genetic mapping; Monocotyledones; Molecular marker; Gene expression; Restriction fragment length polymorphism; Gene; Gramineae; Angiospermae; Genetics; Wild plant; Spermatophyta; Hordeum bulbosum; Comparative study
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-002-0932-2

A cDNA sequence (Hbc8-2) isolated from pistils of the self-incompatible species Hordeum bulbosum was analysed for expression pattern and genetic map location. Hbc8-2 was expressed just prior to anthesis in mature pistils, and expression was maintained at a high level throughout anthesis. The same expression pattern was found in self-incompatible rye ( Secale cereale), but no expression was detected in the self-compatible cereals wheat ( Triticum aestivum) or barley ( Hordeum vulgare) at comparable stages of development. However, three wheat expressed sequence tags from a pre-anthesis library had high homology to Hbc8-2. Southern blot analyses using Hbc8-2 as a probe detected hybridising bands in the genomes of various Gramineae species including rye, barley, bread wheat, wild wheat relatives ( Aegilops tauschii and Ae. speltoides), oats ( Avena fatua and A. strigosa), rice ( Oryza sativa) and maize ( Zea mays). This suggests that Hbc8-2-like sequences are present in many species but that high levels of expression may be associated with self-incompatibility. Hbc8-2 was mapped on the long arms of chromosome 2H(b) of H. bulbosum, 2R of rye, and 2B and 2D of wheat and was assigned to chromosome 2H of barley using wheat/barley addition lines. On a H. bulbosum genetic map, Xhbc8-2 was located between Xbcd266 and Xpsr87, while in rye and wheat it was located in a 13.2-cM interval between Xpsr331 and Xpsr932, consistent with previous comparative mapping studies of these species. Mapping in rye suggested that Hbc8-2 is probably proximal to the Z self-incompatibility locus which was previously shown to be tightly linked to Xbcd266.