An investigation of the degradation of the plant toxin, ricin, by sodium hypochlorite
The toxin, ricin (0.4 μg/μl), was exposed to a range of sodium hypochlorite concentrations. SDS PAGE showed that hypochlorite caused the ricin to smear and decrease in mobility and, ultimately, caused a loss of silver staining. Cytotoxicity assays using dye uptake by Hep2 cells showed that treatment...
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Published in | Toxicon (Oxford) Vol. 38; no. 2; pp. 287 - 291 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier Ltd
01.02.2000
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | The toxin, ricin (0.4 μg/μl), was exposed to a range of sodium hypochlorite concentrations. SDS PAGE showed that hypochlorite caused the ricin to smear and decrease in mobility and, ultimately, caused a loss of silver staining. Cytotoxicity assays using dye uptake by Hep2 cells showed that treatment with 3 mM hypochlorite inactivated the ricin. Western blotting and ELISAs showed that binding by polyclonal antibodies raised against native ricin, or partially degraded ricin, diminished as hypochlorite degradation of the ricin increased. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0041-0101 1879-3150 |
DOI: | 10.1016/S0041-0101(99)00142-7 |