An investigation of the degradation of the plant toxin, ricin, by sodium hypochlorite

The toxin, ricin (0.4 μg/μl), was exposed to a range of sodium hypochlorite concentrations. SDS PAGE showed that hypochlorite caused the ricin to smear and decrease in mobility and, ultimately, caused a loss of silver staining. Cytotoxicity assays using dye uptake by Hep2 cells showed that treatment...

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Published inToxicon (Oxford) Vol. 38; no. 2; pp. 287 - 291
Main Authors Mackinnon, Philip J, Alderton, Malcolm R
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.02.2000
Elsevier Science
Subjects
Animals
Biological and medical sciences
Disinfectants - pharmacology
Electrophoresis, Polyacrylamide Gel
Female
Medical sciences
Mice
Mice, Inbred BALB C
Plant poisons toxicology
Ricin - immunology
Ricin - metabolism
Ricin - toxicity
sodium hypochlorite
Sodium Hypochlorite - pharmacology
Toxicology
Destruction
Ricin
Toxicity
In vitro
Degradation
Chemical warfare agent
Toxin
Decontamination
Dicotyledones
Angiospermae
Plant origin
Euphorbiaceae
Spermatophyta
Ricinus communis
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Summary:The toxin, ricin (0.4 μg/μl), was exposed to a range of sodium hypochlorite concentrations. SDS PAGE showed that hypochlorite caused the ricin to smear and decrease in mobility and, ultimately, caused a loss of silver staining. Cytotoxicity assays using dye uptake by Hep2 cells showed that treatment with 3 mM hypochlorite inactivated the ricin. Western blotting and ELISAs showed that binding by polyclonal antibodies raised against native ricin, or partially degraded ricin, diminished as hypochlorite degradation of the ricin increased.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0041-0101
1879-3150
DOI:10.1016/S0041-0101(99)00142-7