Decreased expression of annexin A1 is correlated with breast cancer development and progression as determined by a tissue microarray analysis

• Published in: Human pathology Vol. 37; no. 12; pp. 1583 - 1591
• Main Authors: Shen, Dejun, Nooraie, Farzad, Elshimali, Yahya, Lonsberry, Victor, He, Jianbo, Bose, Shikha, Chia, David, Seligson, David, Chang, Helena R., Goodglick, Lee
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.2006; Elsevier; Elsevier Limited
• Subjects: Annexin A1; Annexin A1 - biosynthesis; Apoptosis; Biological and medical sciences; Breast - metabolism; Breast - pathology; Breast cancer; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Carcinoma, Ductal, Breast - metabolism; Carcinoma, Ductal, Breast - pathology; Carcinoma, Intraductal, Noninfiltrating - metabolism; Carcinoma, Intraductal, Noninfiltrating - pathology; Cell culture; Disease Progression; Gynecology. Andrology. Obstetrics; Histology; Humans; Hyperplasia - metabolism; Immunohistochemistry; Investigative techniques, diagnostic techniques (general aspects); Kinases; Lymphatic Metastasis - pathology; Lymphoma; Mammary gland diseases; Medical sciences; Myoepithelium; Oligonucleotide Array Sequence Analysis; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Prostate cancer; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Signal transduction; Studies; Tissue microarray; Tumor marker; Tumors; Tumor marker; Tissue microarray; Breast cancer; Annexin A1; Myoepithelium; Mammary gland diseases; Annexin Al; Anatomic pathology; Tissue microarrays; Tumor progression; Tumoral marker; Malignant tumor; Carcinogenesis
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/j.humpath.2006.06.001

Annexin A1 (ANXA1) is a calcium- and phospholipid-binding protein and a known mediator of glucocorticoid-regulated inflammatory responses. Using a combined multiple high-throughput approach, we recently identified a reduced expression of ANXA1 in human breast cancer. The finding was confirmed at the gene level by quantitative reverse transcription-polymerase chain reaction and at the protein level by immunohistochemical staining of normal, benign, and malignant breast tissues. In this study, we constructed and used a high-density human breast cancer tissue microarray to characterize the expressional pattern of ANXA1 according to histopathologies. The tissue microarray contains 1158 informative breast tissue cores of different histologies including normal tissues, hyperplasia, in situ and invasive tumors, and lymph node metastases. Our results showed that there was a significant decrease in glandular expression of ANXA1 in ductal carcinoma in situ and invasive ductal carcinoma compared with either normal breast tissue or hyperplasia ( P < .0001). Moreover, in benign breast tissue, myoepithelial cells showed strong expression of ANXA1. There was a decrease of ANXA1 expression in myoepithelial cells in ductal carcinoma in situ lesions compared with the same cell population in either normal or hyperplastic lesions. These results suggest that suppressed ANXA1 expression in breast tissue is correlated with breast cancer development and progression.