An investigation of electrospun Henna leaves extract-loaded chitosan based nanofibrous mats for skin tissue engineering

• Published in: Materials Science & Engineering C Vol. 75; pp. 433 - 444
• Main Authors: Yousefi, Iman, Pakravan, Mehdi, Rahimi, Hoda, Bahador, Abbas, Farshadzadeh, Zahra, Haririan, Ismael
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2017; Elsevier BV
• Subjects: Animals; Antibacterial activity; Bacteria; Biocompatibility; Biodegradability; Biodegradation; Biomedical materials; Cell proliferation; Chitosan; Chitosan - chemistry; Chitosan - pharmacology; Electrospinning; Fibers; Fourier transforms; Henna extract; Humans; In vivo methods and tests; Infrared spectroscopy; Lawsonia inermis; Lawsonia Plant - chemistry; Leaves; Male; Materials science; Mats; Mechanical properties; Medical dressings; Nanofiber; Nanofibers; Nanofibers - chemistry; Plant extracts; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Rats; Rats, Wistar; Scaffolds; Skin; Skin, Artificial; Synergistic effect; Tissue engineering; Tissue Engineering - methods; Wound healing; Electrospinning; Lawsonia inermis; Nanofiber; Wound healing; Chitosan; Henna extract
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2017.02.076

Wound healing characteristics of some plant extracts have been well known for many years, and they have been utilized for such applications in traditional way. Recently electrospun nanofibrous mats showed promising properties for tissue engineering and especially for skin repair. It is expected that incorporation of plant extracts into such structures could provide higher performance and synergistic effect for biomedical and wound healing applications. The final purpose of this study is to fabricate chitosan based nanofiber mats loaded with a traditional plant extract of Lawsonia inermis (Henna) leaves to enhance the antibacterial efficacy and wound healing of the precursor nanofibers. The morphology, structure, mechanical properties and swelling and weight loss degree of the electrospun nanofibers have been investigated in this study. Antibacterial activity, cell biocompatibility evaluations and in vivo wound healing activity of the abovementioned mats were also studied. The FESEM images of Henna leaves extract-loaded nanofibers proved that homogeneous, smooth and defect free nanofibers of 64–87nm in diameter have been prepared. Presence of Henna extract in the electrospun fibers was approved by Fourier Transform Infrared spectroscopy. Incorporation of Henna extract into the nanofiber mats exhibited significant synergistic antibacterial activity against bacterial cells. It was well supported by the results of cell viability and proliferation of human foreskin fibroblast cells on the prepared scaffolds. Therefore, the results of this work showed that Henna leaves extract incorporated chitosan nonwoven mats have a great potential to be used as the biodegradable, biobased and antibacterial wound healing dressings. [Display omitted] •Henna leaves extract were successfully loaded into chitosan based nanofiber mats.•These mats demonstrated significant synergistic antibacterial activity.•Combined properties of chitosan nanofibers and Henna promoted cell viability.•The prepared nonwoven mats exhibited outstanding wound healing activities.•These nanofibrous mats showed a great potential in skin tissue engineering.