CRISPR/Cas12a-mediated liposome-amplified strategy for the photoelectrochemical detection of nucleic acid

• Published in: Chemical communications (Cambridge, England) Vol. 57; no. 71; pp. 8977 - 898
• Main Authors: Gong, Hexiang, Wu, Yulin, Zeng, Ruijin, Zeng, Yongyi, Liu, Xiaolong, Tang, Dianping
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 06.09.2021
• Subjects: Amplification; Beads; Biosensors; CRISPR; Deoxyribonucleic acid; DNA; Dopamine; Human papillomavirus; Liposomes; Magnetic separation; Neodymium; Nucleic acids; Photoelectric effect; Photoelectric emission
• ISSN: 1359-7345; 1364-548X
• DOI: 10.1039/d1cc03743a

This study reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas 12a system for the quantification of human papilloma virus (HPV)-related DNA using neodymium-doped BiOBr nanosheets (Nd-BiOBr) as a photoactive matrix. Magnetic beads and dopamine-loaded liposomes are covalently attached to the both ends of ssDNA to construct dumbbell-shaped dopamine-loaded liposome-encoded magnetic bead (DLL-MB) probes. When the guide RNA binds to the target HPV-16, the ssDNA will be cleaved by Cas12a, thereby degrading the double dumbbell probes. After magnetic separation, the dissolved DLLs are treated with Triton X-100 to release the dopamine (as an electron donor), which was then detected by an amplified photocurrent using the Nd-BiOBr-based photoelectrode. This study reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by CRISPR/Cas 12a system for the quantification of human papilloma virus-related DNA using neodymium-doped BiOBr nanosheets.