FXII levels, FXIIa-like activities and kallikrein activities in normal subjects and patients undergoing cardiac surgery

• Published in: Immunopharmacology Vol. 45; no. 1-3; pp. 141 - 144
• Main Authors: Wendel, H.P, Jones, D.W, Gallimore, M.J
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.1999; Elsevier
• Subjects: Adult; Aged; Biological and medical sciences; Blood coagulation. Blood cells; Cardiac surgery; Coagulation factors; Coronary Artery Bypass; Enzyme Activation; Factor XII; Factor XII - metabolism; Factor XIIa - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Kallikrein; Kallikreins - metabolism; Male; Middle Aged; Molecular and cellular biology; Perioperative Care; Kallikrein; Factor XII; Cardiac surgery; Heart; Human; Serine endopeptidases; Enzyme; Cardiovascular disease; Activation; Chromogenic substrate; Blood plasma; Peptidases; Coagulation Factor XIIa; Surgery; Hydrolases; Coagulation factor
• ISSN: 0162-3109
• DOI: 10.1016/S0162-3109(99)00067-3

A new chromogenic peptide substrate assay kit was used to measure factor XII (FXII) levels in plasma samples from 115 male patients with heart disease awaiting cardiac surgery, 40 age-matched normal healthy male blood donors and 20 patients before, during and after cardiopulmonary bypass surgery (CPB). Kallikrein-like and FXIIa-like activities were also determined in the CPB patient group. FXII levels were significantly lower (p=0.0049) in the heart disease patients awaiting surgery when compared with values for the healthy donors and 13 patients (11.3%) had FXII levels below 50% compared with 1 normal donor (2,5%). During CPB significant decreases in FXII levels and significant increases in FXIIa-like and kallikrein-like activities were found indicating activation of the FXII-plasma kallikrein pathway during CPB.