Prokaryotic expression of antibodies

Maximizing the expression yields of recombinant whole antibodies and antibody fragments such as Fabs, single-chain Fvs and single-domain antibodies is highly desirable since it leads to lower production costs. Various eukaryotic and prokaryotic expression systems have been exploited to accommodate a...

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Bibliographic Details
Published inCancer and metastasis reviews Vol. 24; no. 4; pp. 501 - 519
Main Authors Arbabi-Ghahroudi, Mehdi, Tanha, Jamshid, MacKenzie, Roger
Format Journal Article
LanguageEnglish
Published Netherlands Springer Nature B.V 01.12.2005
Subjects
Animals
Antibodies - genetics
Antibodies - metabolism
Antigen-Antibody Complex
Escherichia coli
Escherichia coli - genetics
Escherichia coli - immunology
Escherichia coli - metabolism
Humans
Immunoglobulin Fragments - biosynthesis
Immunoglobulin Fragments - genetics
Immunohistochemistry
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Recombinant Proteins - isolation & purification
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Summary:Maximizing the expression yields of recombinant whole antibodies and antibody fragments such as Fabs, single-chain Fvs and single-domain antibodies is highly desirable since it leads to lower production costs. Various eukaryotic and prokaryotic expression systems have been exploited to accommodate antibody expression but Escherichia coli systems have enjoyed popularity, in particular with respect to antibody fragments, because of their low cost and convenience. In many instances, product yields have been less than adequate and intrinsic and extrinsic variables have been investigated in an effort to improve yields. This review deals with various aspects of antibody expression in E. coli with a particular focus on single-domain antibodies.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
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ObjectType-Review-1
ISSN:0167-7659
1573-7233
DOI:10.1007/s10555-005-6193-1