Comparison of UV-induced unscheduled DNA synthesis in lymphocytes exposed to low doses of ionising radiation in vivo and in vitro

• Published in: Mutation research Vol. 447; no. 2; p. 199
• Main Authors: Mohankumar, M N, Venkatachalam, P, Prabhu, B K, Jeevanram, R K
• Format: Journal Article
• Language: English
• Published: Netherlands 14.02.2000
• Subjects: Adult; Cells, Cultured; DNA - biosynthesis; DNA - radiation effects; DNA Repair - radiation effects; Female; Humans; Lymphocytes - cytology; Lymphocytes - metabolism; Lymphocytes - radiation effects; Male; Middle Aged; Occupational Exposure - adverse effects; Radiation Dosage; Radiation, Ionizing; Thymidine - metabolism; Tritium; Ultraviolet Rays
• ISSN: 0027-5107
• DOI: 10.1016/S0027-5107(99)00207-9

In an attempt to understand and ascertain the stimulatory effects of low-dose ionising radiation, a study was conducted to compare the changes in the UV-induced repair capacity of human blood cells exposed to low conditioning doses of ionising radiation under in vivo and in vitro conditions. A significant increase in the rate of UV induced Unscheduled DNA synthesis (UDS) in lymphocytes pre-exposed to low doses of ionising radiation was observed both under in vitro and in vivo conditions. There was also a significant correlation between the adapting dose and net UDS in lymphocytes of radiation workers implying that the triggering action of the adaptation process is dose dependent. However, on comparing the extent of UV-induced UDS of the in vivo and in vitro exposures, significantly higher rates of UDS were observed in the lymphocytes of radiation workers when compared to a corresponding in vitro adapting dose. We postulate that the response in vivo is much more pronounced due to cell repopulating events and extra cellular secretory factors like hormones etc,.