Central l-proline attenuates stress-induced dopamine and serotonin metabolism in the chick forebrain

• Published in: Neuroscience letters Vol. 460; no. 1; pp. 78 - 81
• Main Authors: Hamasu, Kousuke, Shigemi, Kazutaka, Kabuki, Yusuke, Tomonaga, Shozo, Denbow, D. Michael, Furuse, Mitsuhiro
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 21.08.2009; Elsevier
• Subjects: Analysis of Variance; Animals; Animals, Newborn; Behavior, Animal; Biological and medical sciences; Chick; Chickens; Chromatography, High Pressure Liquid - methods; Disease Models, Animal; Dopamine; Dopamine - metabolism; Drug Administration Routes; Electrochemistry - methods; Fundamental and applied biological sciences. Psychology; Homovanillic Acid - metabolism; Hydroxyindoleacetic Acid - metabolism; l-Proline; Male; Microdialysis; Microdialysis - methods; NMDA; Proline - administration & dosage; Restraint, Physical - methods; Serotonin - metabolism; Stress; Stress, Psychological - drug therapy; Stress, Psychological - etiology; Stress, Psychological - metabolism; Vertebrates: nervous system and sense organs; NMDA; Microdialysis; l-Proline; Chick; Stress; Dopamine; Serotonin; Central nervous system; Proline; Catecholamine; Metabolism; Prosencephalon; L-Proline; Vertebrata; Animal; Neurotransmitter; Chicken; Aves
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2009.05.036

Using microdialysis, we investigated the effect of l-proline on monoamine release in the medio-rostral neostriatum/hyperstriatum ventrale (MNH) of freely moving and restricted chicks. A 30 min handling-stress resulted in a significant increase in extracellular homovallinic acid (HVA), a dopamine metabolite, and 5-hydroxyindoleacetic acid (5-HIAA), a serotonin metabolite, in the MNH. l-Proline, perfused through the microdialysis probe into the MNH during the stressed condition, significantly attenuated the average dialysate concentration of HVA produced by handling-stress. Handling-stress resulted in a significant increase in 5-HIAA levels in the control group, which were attenuated by profusion with l-proline. l-Proline did not significantly modify basal concentrations of HVA or 5-HIAA in the MNH during control conditions. These results show that perfusion of l-proline modified the turnover/metabolism of dopamine and serotonin in the MNH caused by handling-stress.