Electrophoretic Determination of Trimethylamine (TMA) in Biological Samples as a Novel Potential Biomarker of Cardiovascular Diseases Methodological Approach

• Published in: International journal of environmental research and public health Vol. 18; no. 23; p. 12318
• Main Authors: Konop, Marek, Rybka, Mateusz, Waraksa, Emilia, Laskowska, Anna K, Nowiński, Artur, Grzywacz, Tomasz, Karwowski, Wojciech J, Drapała, Adrian, Kłodzińska, Ewa Maria
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.11.2021; MDPI
• Subjects: Athletes; Bioavailability; Biological properties; Biological samples; Biomarkers; Capillary electrophoresis; Cardiovascular diseases; Cardiovascular Diseases - diagnosis; Carnitine; Caustic soda; Choline; Chromatography; Circulatory system; Colon; Concept Paper; Coronary artery disease; Diet; Differential diagnosis; Disease; Eggs; Electric fields; Electrophoresis; Gastrointestinal Microbiome; Heart; Heart diseases; High-throughput screening; Humans; Intestinal microflora; Laboratories; Meat; Metabolites; Methylamines; Microbiota; Trimethylamine; Veganism; Vegetarianism; Poland; capillary electrophoresis (CE); determination of trimethylamine (TMA); biomarkers; cardiovascular diseases in athletes
• ISSN: 1660-4601; 1661-7827; 1660-4601
• DOI: 10.3390/ijerph182312318

In competitive athletes, the differential diagnosis between nonpathological changes in cardiac morphology associated with training (commonly referred to as "athlete's heart") and certain cardiac diseases with the potential for sudden death is an important and not uncommon clinical problem. The use of noninvasive, fast, and cheap analytical techniques can help in making diagnostic differentiation and planning subsequent clinical strategies. Recent studies have demonstrated the role of gut microbiota and their metabolites in the onset and the development of cardiovascular diseases. Trimethylamine (TMA), a gut bacteria metabolite consisting of carnitine and choline, has recently emerged as a potentially toxic molecule to the circulatory system. The present work aims to develop a simple and cost-effective capillary electrophoresis-based method for the determination of TMA in biological samples. Analytical characteristics of the proposed method were evaluated through the study of its linearity (R > 0.9950) and the limit of detection and quantification (LOD = 1.2 µg/mL; LOQ = 3.6 µg/mL). The method shows great potential in high-throughput screening applications for TMA analysis in biological samples as a novel potential biomarker of cardiovascular diseases. The proposed electrophoretic method for the determination of TMA in biological samples from patients with cardiac disease is now in progress.