Genomic organisation and expression of BCL6 in murine B-cell lymphomas

• Published in: Leukemia research Vol. 24; no. 8; pp. 719 - 732
• Main Authors: Qi, Chen-Feng, Hori, Mitsuo, Coleman, Allen E, Torrey, Ted A, Taddesse-Heath, Lekidelu, Ye, B.Hilda, Chattopadhyay, Sisir K, Hartley, Janet W, Morse III, H.C
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.08.2000; Elsevier Science
• Subjects: Animal tumors. Experimental tumors; Animals; Bcl-6 protein; BCL6; Biological and medical sciences; chromosome 16; Chromosome Mapping; DNA-Binding Proteins - genetics; Genome; Humans; Karyotyping; Lymphoma, B-Cell - genetics; Medical sciences; Mice; Mouse B-cell lymphoma; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins c-bcl-6; Spectral karyotyping; Spontaneous animal tumors; Transcription Factors - genetics; Translocation; Tumor Cells, Cultured; Tumors; Zinc Fingers; Translocation; DLCL(LL), lymphoblastic lymphoma-like DLCL; DLCL(IB), immunoblastic type DLCL; GC, germinal centre; Spectral karyotyping; MZL, splenic marginal zone lymphoma; PBS, phosphate buffered saline; SLL, small lymphocytic lymphoma; FL, follicular lymphoma; DLCL(CB), centroblastic type DLCL; MuLV, murine leukaemia virus; BCL6; Mouse B-cell lymphoma; FISH, fluorescence in situ hybridisation; DLCL, diffuse large cell B lymphoma; SKY, spectral karyotyping; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Chromosomal aberration; Rodentia; Malignant hemopathy; B-Lymphocyte; Gene expression; Carcinogenesis; Lymphoma; Vertebrata; Mammalia; Abnormal chromosome B5; Mouse; Abnormal chromosome E16; Lymphoproliferative syndrome; Animal; C-Onc gene; Cytogenetics; Abnormal chromosome; Genetics; Protooncogene; Chromosome translocation
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/S0145-2126(00)00028-X

BCL6 encodes a transcription factor deregulated by chromosomal translocations in human diffuse large cell B lymphomas (DLCL). This study was designed to determine whether Bcl6 might also be involved in lymphomas of mice. BCL6 protein was expressed at high levels in 90% or more of DLCL but not in low grade B lymphomas. Southern hybridisation studies demonstrated altered organisation of Bcl6 in three primary DLCL and the WEHI 231 B-cell lymphoma cell line but not in low grade tumours. Chromosomal painting and fluorescence in situ hybridisation (FISH) analyses of the WEHI 231 metaphase spreads revealed a T(5;16) translocation with Bcl6 on Chromosome 16 at the translocation breakpoint. Deregulated expression of BCL6 is thus likely to contribute to the genesis of DLCL of mice as well as of humans.