Selective ex vivo expansion of cytomegalovirus‐specific CD4+ and CD8+ T lymphocytes using dendritic cells pulsed with a human leucocyte antigen A0201‐restricted peptide

Adoptive transfer of ex vivo‐generated cytomegalovirus (CMV)‐specific T lymphocytes may be effective in preventing CMV disease in allogeneic haematopoietic stem cell transplantation (HSCT) recipients. We developed a procedure for expansion of CMV‐specific T lymphocytes based on the antigen‐presentin...

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Bibliographic Details
Published inBritish journal of haematology Vol. 113; no. 2; pp. 479 - 482
Main Authors Vannucchi, Alessandro M., Glinz, Stephanie, Bosi, Alberto, Caporale, Roberto, Rossi‐Ferrini, Pierluigi
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Science Ltd 01.05.2001
Blackwell
Blackwell Publishing Ltd
Subjects
adoptive immunity
Adoptive Transfer - methods
Biological and medical sciences
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
cytomegalovirus
Cytomegalovirus - immunology
dendritic cells
Dendritic Cells - immunology
Hematology
HLA-A Antigens - immunology
Humans
Immunopathology
Immunotherapy (general aspects)
Interferon-gamma - metabolism
Medical sciences
peptide
T clones
T-Lymphocytes - immunology
Human
Dendritic cell
Cell culture
Cytomegalovirus
Antigen presentation
Immunostimulation
Cell therapy
Herpesviridae
Betaherpesvirinae
Adoptive transfer
Virus
Infection
Treatment
Ex vivo
Antigen presenting cell
Immunotherapy
T-Lymphocyte
Expansion
Clone
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Summary:Adoptive transfer of ex vivo‐generated cytomegalovirus (CMV)‐specific T lymphocytes may be effective in preventing CMV disease in allogeneic haematopoietic stem cell transplantation (HSCT) recipients. We developed a procedure for expansion of CMV‐specific T lymphocytes based on the antigen‐presenting function of donor dendritic cells (DCs), pulsed with a human leucocyte antigen A*0201‐restricted pp65 nonamer peptide. CMV‐specific T lymphocytes were identified following induction of interferon γ (IFN‐γ) secretion prompted by peptide exposure. Both CD8+ and CD4+ CMV‐specific T lymphocytes were selectively produced in these cultures and showed CMV‐restricted cytotoxicity. The simultaneous and selective expansion of CD4+ and CD8+ CMV‐specific lymphocytes might be instrumental for more efficient in vivo function of infused CMV‐specific lymphocytes.
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ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.2001.02777.x