Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI

• Published in: Yeast (Chichester, England) Vol. 12; no. 1; pp. 77 - 84
• Main Authors: Naitou, Masanori, Ozawa, Masashi, Sasanuma, Syun‐Ichi, Kobayashi, Motoe, Hagiwara, Hiroko, Shibata, Takehiko, Hanaoka, Fumio, Watanabe, Koji, Ono, Ayako, Yamazaki, Masaaki, Tashiro, Hiroyuki, Eki, Toshihiko, Murakami, Yasufumi
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 1996
• Subjects: Amino Acid Sequence; Base Sequence; Chromosome Mapping; chromosome VI; Chromosomes, Fungal - genetics; DEG1; DNA, Fungal - genetics; Gene Expression; Genes, Fungal; Molecular Sequence Data; MSH4; NIC96; Open Reading Frames; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; SEC4; SPB4; TYA; TYB
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/(SICI)1097-0061(199601)12:1<77::AID-YEA887>3.0.CO;2-5

Plasmid clone gapB and lambda phage clone 4682, which contain fragments of Saccharomyces cerevisiae chromosome VI, were analysed. A 23 kb sequence was determined and ten open reading frames (ORFs) were revealed. Among them, five ORFs were identical to five yeast genes (SEC4, MSH4, SPB4, DEG1 and NIC96), two were identical to transposable elements (TYA and TYB), one (gapBorfF003) was highly homologous to a yeast expressed sequence tag, and another (4682orfF002) was predicted to be a nuclear protein. Sequence data have been submitted to DDBJ/EMBL/GenBank data library under Accession Number D44604 (clone gapB) and D44600 (clone 4682), respectively.