Plasma esterase-1 (ES-1) activity is increased in rats fed high-fat diets

• Published in: Lipids Vol. 24; no. 1; pp. 86 - 88
• Main Authors: Lith, H.A. van, Meijer, G.W, Zutphen, L.F.M. van, Beynen, A.C
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer‐Verlag 01.01.1989; Springer
• Subjects: ACEITE DE COCO; ACEITES VEGETALES; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Afdeling Humane voeding; Animals; Biological and medical sciences; BLOOD PLASMA; Carboxylesterase; Carboxylic Ester Hydrolases - blood; COCONUT OIL; Corn Oil - pharmacology; CORPS GRAS; Dietary Fats - pharmacology; Dose-Response Relationship, Drug; Enzyme Activation - drug effects; ENZYMIC ACTIVITY; ESTERASAS; ESTERASE; ESTERASES; FATS; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; GRASAS; HUILE DE COPRAH; HUILE VEGETALE; Human Nutrition; Human Nutrition & Health; Human Nutrition (HNE); Humane Voeding; Humane Voeding & Gezondheid; Male; PLANT OILS; PLASMA SANGUIN; PLASMA SANGUINEO; RAT; RATA; RATS; Vertebrates: anatomy and physiology, studies on body, several organs or systems; ZEA MAYS; Rat; Gel electrophoresis; Enzyme; Isozyme; Rodentia; Lipids; Vegetal oil; Feeding; Blood plasma; Vertebrata; Mammalia; Enzymatic activity; Esterase; Diet; Fat
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/BF02535271

The question addressed is whether the amount and type of dietary fat affects esterases in plasma. Rats were fed semipurified diets containing 2.0 to 19.4% (w/w) of fat in the form of coconut fat or corn oil. Fat was added to the diets at the expense of isocaloric amounts of carbohydrates. Plasma total esterase activities measured with 4‐nitrophenylacetate as substrate were slightly increased with increasing fat intakes. However, an increase in fat concentration of the diet was associated with a pronounced increase in the activity of the so‐called ES‐1 isozyme in plasma. ES‐1, which represents very little plasma total esterase activity, was quantified densitometrically as the high‐mobility, anodal esterase band on polyacrylamide gel electrophoresis. The positive association between amount of dietary fat and ES‐1 activity was identical for coconut fat and corn oil.