Fluorescence Detection of Specific RNA Sequences Using 2′-Pyrene-Modified Oligoribonucleotides

Labeling the 2′‐hydroxy function of RNA with a pyrenylmethyl group (Pyr) provides a useful probe of RNA because of its high fluorescent quantum efficiency on binding. The fluorescence exhibits sequence specificity and binds with a high affinity (see schematic representation).

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Bibliographic Details
Published inAngewandte Chemie International Edition Vol. 40; no. 6; pp. 1104 - 1106
Main Authors Yamana, Kazushige, Zako, Hirofumi, Asazuma, Kentarou, Iwase, Reiko, Nakano, Hidehiko, Murakami, Akira
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag GmbH 16.03.2001
WILEY‐VCH Verlag GmbH
Subjects
fluorescence
oligonucleotides
RNA recognition
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Summary:Labeling the 2′‐hydroxy function of RNA with a pyrenylmethyl group (Pyr) provides a useful probe of RNA because of its high fluorescent quantum efficiency on binding. The fluorescence exhibits sequence specificity and binds with a high affinity (see schematic representation).
Bibliography:istex:2641E756A082ADF1B94C75808D94021905E8DA2B
ArticleID:ANIE11040
ark:/67375/WNG-RR7ZNSZJ-5
We are very grateful to Professor Hiroshi Sugiyama, Dr. Tetsuji Yamaoka, and Dr. Takashi Morii for ion‐spray mass spectrometric measurements and helpful comments on this research.
ISSN:1433-7851
1521-3773
DOI:10.1002/1521-3773(20010316)40:6<1104::AID-ANIE11040>3.0.CO;2-2