Myelin protein Po as a potential autoantigen in autoimmune inner ear disease

We have previously shown that a 30,000 Mr protein extracted from guinea pig inner ear tissue is recognized by autoantibodies present in the serum of patients suffering from autoimmune inner ear disease. This protein was localized in the modiolus and in the organ of Corti. We have now identified this...

Full description

Saved in:
Bibliographic Details
Published inThe FASEB journal Vol. 10; no. 14; p. 1635
Main Authors Cao, M Y, Dupriez, V J, Rider, M H, Deggouj, N, Gersdorff, M C, Rousseau, G G, Tomasi, J P
Format Journal Article
LanguageEnglish
Published United States 01.12.1996
Subjects
Amino Acid Sequence
Animals
Autoantigens - immunology
Autoimmune Diseases - immunology
Ear Diseases - immunology
Ear, Middle - immunology
Female
Guinea Pigs
Humans
Male
Molecular Sequence Data
Myelin P0 Protein - immunology
Peptide Mapping
Online AccessGet more information

Cover

More Information
Summary:We have previously shown that a 30,000 Mr protein extracted from guinea pig inner ear tissue is recognized by autoantibodies present in the serum of patients suffering from autoimmune inner ear disease. This protein was localized in the modiolus and in the organ of Corti. We have now identified this protein by a combination of microsequencing and matrix-assisted laser desorption ionization time-of-flight mass spectrometry of its tryptic peptides. A partial sequence of the protein was thereby determined. These data and 2-dimensional gel electrophoresis followed by immunoblotting experiments showed that the 30,000 Mr inner ear antigen is the major peripheral myelin protein Po. This suggests that protein Po may be an important autoantigen in autoimmune inner ear disease.
ISSN:0892-6638
DOI:10.1096/fasebj.10.14.9002556